This project aims to quantify the resiliency of prairie ecosystems in the U.S. Pacific Northwest (PNW) to climate change. Prairies in this region sustain over one million beef cows, and cow­-calf production costs are expected to increase to offset warming-induced plant productivity loss. We investigated the above- and belowground effects of experimental warming in prairie ecosystems by assessing biogeochemical controls on and patterns of asymbiotic nitrogen fixation (ANF), plant species diversity, and legume cover to address a major challenge for sustainable agriculture in the region. We hypothesize that the effect of warming on prairie functional diversity increases soil asymbiotic nitrogen inputs by decreasing legume cover and soil nitrogen availability. We quantified the effects of decadal warming stress (+2.5ºC) on soil biogeochemical properties and plant species and functional diversity during fall and spring seasons in three sites along a 520­km latitudinal gradient—from central Washington to southern Oregon—representing a drought severity gradient. At each site, we collected composite soil samples from five co-­located prairie plots under control (ambient) and warming conditions. We incubated these soils using 15N-labeled dinitrogen (15N2), and quantified total soil carbon­, total and available nitrogen, and available phosphorus and iron pools to better understand the underlying mechanisms governing warming-­induced changes in ANF. We used a point intercept technique to survey plot-level plant community composition and calculate Shannon’s diversity index and percent cover of legumes (members of Fabaceae according to the Integrated Taxonomic Information System). Warming significantly decreased plant species diversity which also decreased along the drought severity gradient. Legume cover significantly increased from 3.1% in the north to 9.2% in the south. ANF response to warming varied by season and site, where rates increased with the drought severity gradient in the fall but decreased during the spring. Total soil inorganic nitrogen availability was the strongest predictor of ANF response to warming in the spring but not in the fall. Our study highlights the importance of using soil-plant-atmosphere interactions to assess prairie ecosystem resilience to climate change in the PNW.

Danthonia californica is a native perennial bunchgrass commonly used in the restoration of prairie ecosystems in the western United States. Plants of this species simultaneously produce both chasmogamous (potentially outcrossed) and cleistogamous (obligately self-fertilized) seeds. Restoration practitioners almost exclusively use chasmogamous seeds for outplanting, which are predicted to perform better in novel environments due to their greater genetic diversity. Meanwhile, cleistogamous seeds may exhibit greater local adaptation to the conditions in which the maternal plant exists. We performed a common garden experiment at two sites in the Willamette Valley, Oregon to assess the influence of seed type and source population (eight populations) on germination and found no evidence of local adaptation for either seed type. Cleistogamous seeds outperformed chasmogamous seeds regardless of whether seeds were sourced directly from the common gardens (local seeds), or other populations (nonlocal seeds). Furthermore, average seed weight had a strong positive effect on germination success, despite the fact that chasmogamous seeds had significantly greater mass than cleistogamous seeds. At one common garden we observed that seeds of both types sourced from north of our planting site performed significantly better than local or southern-sourced seeds. We also found a significant seed type and distance-dependent interaction, with cleistogamous germination peaking approximately 125km from the garden, which may be explained by differences in the pathogen content of cleistogamous and chasmogamous seeds. These results suggest that cleistogamous seeds should be considered for greater use in D. californica restoration.