Pigeon aviadenovirus A and Pigeon circovirus are both viruses that can cause disease in pigeons. This study reports the identification of a natural co-infection of Pigeon aviadenovirus A and Pigeon circovirus in a breeding pigeon flock in central Anatolia, Turkey. Both viruses were isolated from pooled internal organs of pigeons using primary chicken embryo kidney cell cultures (CEKC) and specific pathogen free (SPF) embryonated chicken eggs. Both viruses were identified by PCR amplification followed by Sanger sequencing, while histopathological examination showed degenerated hepatocytes with basophilic intranuclear viral inclusions. The viruses have similar transmission characteristics and common clinical manifestations, but it is possible that coinfection may exacerabate disease. This is the first report of its kind in Turkey and is important for the protection against disease in pigeons.