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PEYER'S PATCHES, BISPHENOL A INGESTION AND SELENIUM SUPPLEMENTATION IN MURINE MODELS.
  • +6
  • João Araújo,
  • Clarisse Fonseca,
  • Marcos Paulo Carvalho Castro,
  • Bianca Mickaela Santos Chaves,
  • Ulisses de Sousa Rigon,
  • Jordana Fonseca Reis,
  • Sergio Guerra,
  • Kelly Palombit,
  • Airton Conde Júnior
João Araújo
Universidade Federal do Piaui

Corresponding Author:[email protected]

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Clarisse Fonseca
Universidade Federal do Piaui
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Marcos Paulo Carvalho Castro
Universidade Federal do Piaui
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Bianca Mickaela Santos Chaves
Universidade Federal do Piaui
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Ulisses de Sousa Rigon
Universidade Federal de Piaui Departamento de Medicina
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Jordana Fonseca Reis
Universidade Federal de Piaui Departamento de Medicina
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Sergio Guerra
Universidade Federal do Piaui
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Kelly Palombit
Universidade Federal do Piaui
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Airton Conde Júnior
Universidade Federal do Piaui
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Abstract

Bisphenol A [2,2-bis(4hydroxyphenyl) propane, BPA], one of the endocrine disruptors, is of great concern due to its widespread use throughout the world. Several studies have shown that BPA has toxic effects when ingested, making contact with this substance a risk factor for the development of diseases in various organs, including the intestine and associated lymphoid tissue. Therefore, the objective of this work was to study the morphology of Peyer’s patches in young rats supplemented with selenium and exposed to BPA. The study was approved by the UFPI Animal Use Ethics Committee, with protocol number 583/19. 28 male Wistar rats ( Rattus novergicus albinus) were randomly divided into 4 groups: Control (CT), BPA, Se and BPA+Se. The pups were weaned on the 21st day and, from the 22nd postnatal day, the animals in the BPA group received daily doses of 5 mg/kg of BPA diluted in 0.3 ml of corn oil, administered orally. The Se group received 10 μg/kg of Se, the BPA+Se group received 5 mg/kg of BPA and 10 μg/kg of Se and the CT group did not receive any substance but was subjected to the oral gavage process. After 4 weeks of exposure, the puppies were anesthetized and euthanized for intestinal collection and subsequent histological, immunohistochemical and morphometric analysis. Our results showed that BPA caused damage to the epithelial layer of Peyer’s patches, there was a structural disarray in the architecture of the follicular region of the BPA and BPA+Se groups, zones of inflammation with the presence of vacuoles in the tissue. A reduction in Ki-67 expression in Peyer’s patches was also observed in the BPA group, as well as a significant reduction in the number of defense cells in the group. Animals exposed to BPA, but supplemented with Se, showed no damage to the epithelial layer and there was no reduction in Ki-67 expression. BPA, when ingested above the safe level, altered Peyer’s patches tissue, and reduced cell proliferation. Additionally, BPA reduced the immune cell population in Peyer’s patches. Se was able to reverse the observed damage, suggesting antioxidant potential.
29 Oct 2023Submitted to Microscopy Research and Technique
15 Nov 2023Submission Checks Completed
15 Nov 2023Assigned to Editor
19 Nov 2023Review(s) Completed, Editorial Evaluation Pending
19 Nov 2023Reviewer(s) Assigned