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Development of a Novel In Vitro Co-Culture System for Discrete Subaortic Stenosis
  • +3
  • Pengfei Ji,
  • Sunita Brimmer,
  • Swathi Balaji,
  • Jane Grande-Allen,
  • Ravi K. Birla,
  • Sundeep G. Keswani
Pengfei Ji
Texas Children’s Hospital
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Sunita Brimmer
Texas Children’s Hospital
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Swathi Balaji
Texas Children’s Hospital
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Jane Grande-Allen
Rice University
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Ravi K. Birla
Texas Children’s Hospital
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Sundeep G. Keswani
Texas Children’s Hospital

Corresponding Author:[email protected]

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Abstract

The aim of this study was to investigate the survival, distribution and reaction of different cell types on a monolayer disk, as well as their behavior under bioreactor treatment. Specifically, porcine EEC and porcine fibroblasts (PCF) were labeled with GFT and Texas Red, respectively, to track their viability and distribution. The experiments involved monitoring the cells using various microscopy techniques and comparing the results with controls. These findings have important implications for understanding cell behavior and potential applications for Discrete Subaortic Stenosis. This paper aims to discuss the implications of the findings in the context of existing literature and future research directions.
08 Aug 2023Submitted to Biotechnology and Bioengineering
09 Aug 2023Submission Checks Completed
09 Aug 2023Assigned to Editor
09 Aug 2023Review(s) Completed, Editorial Evaluation Pending
14 Aug 2023Reviewer(s) Assigned