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Bio-functionalized titanium surfaces with the modified silk fibroin carrying titanium binding motif enhanced the ossific differentiation of MC3T3-E1
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  • Mai Watanabe,
  • Ujjal Bhawala,
  • Shinji Takemoto,
  • Norihiro Nishiyama,
  • Yuichi Nakahara,
  • Ken-ichiro Tatematsu,
  • Hideki Sezutsu,
  • Nobuo Kuwabara,
  • Tamiko Minamisawa,
  • Kiyotaka Shiba,
  • Tetsuo Asakura
Mai Watanabe
Public Interest Incorporated Foundation Japanese Foundation for Cancer Research

Corresponding Author:[email protected]

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Ujjal Bhawala
Nihon University School of Dentistry Graduate School of Dentistry at Matsudo
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Shinji Takemoto
Iwate Medical University
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Norihiro Nishiyama
Nihon University School of Dentistry Graduate School of Dentistry at Matsudo
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Yuichi Nakahara
National Agriculture and Food Research Organization
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Ken-ichiro Tatematsu
National Agriculture and Food Research Organization
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Hideki Sezutsu
National Agriculture and Food Research Organization
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Nobuo Kuwabara
Gunma Sericultural Technology Center
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Tamiko Minamisawa
Public Interest Incorporated Foundation Japanese Foundation for Cancer Research
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Kiyotaka Shiba
Public Interest Incorporated Foundation Japanese Foundation for Cancer Research
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Tetsuo Asakura
Tokyo University of Agriculture and Technology
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Abstract

Silk fibroin (SF) from Bombyx mori has superior properties as both a textile and a biomaterial, and has been used to functionalize the surfaces of various medical inorganic materials including titanium (Ti). In this paper, we endowed SF with reversible binding ability to Ti by embedding a titanium binding motif (minTBP-1, RKLPDA). Artificial SF proteins were first created by conjugating gene cassettes for SF motif (AGSGAG) and minTBP-1 motif with different ratios, which have been shown to bind reversibly to Ti surfaces in quartz crystal microbalance analyses. Based on these results, the functionalized SF (TiBP-SF) containing the designed peptide [TS[(AGSGAG)3AS]2RKLPDAS]8 was prepared from the cocoon of transgenic B. mori, which accelerates the ossific differentiation of MC3T3-E1 cells when coated on titanium substrates. Thus, TiBP-SF presents an alternative for endowing the surfaces of titanium materials with osseointegration functionality, which would allow the exploration of potential applications in the medical field.
31 Aug 2020Submitted to Biotechnology and Bioengineering
31 Aug 2020Submission Checks Completed
31 Aug 2020Assigned to Editor
10 Oct 2020Reviewer(s) Assigned
19 Mar 2021Editorial Decision: Revise Minor
19 Mar 2021Review(s) Completed, Editorial Evaluation Pending
24 Mar 20211st Revision Received
24 Mar 2021Submission Checks Completed
24 Mar 2021Assigned to Editor
24 Mar 2021Reviewer(s) Assigned
31 Mar 2021Review(s) Completed, Editorial Evaluation Pending
31 Mar 2021Editorial Decision: Accept