Unlocking the formate utilization of wild-type Yarrowia lipolytica
through adaptive laboratory evolution
- qian chen,
- Xue Yang,
- Yunhong Chen,
- Yuyue Ma,
- Jianfeng Huang,
- Zhidan zhang,
- Yefu Chen ,
- Yanfei Zhang,
- Guoping Zhao
Zhidan zhang
Tianjin Institute of Industrial Biotechnology
Author ProfileAbstract
Synthetic biology is contributing to the advancement of the global
net-negative carbon economy, with emphasis on formate as a member of the
one-carbon substrate garnering substantial attention. In this study, we
employed base editing tools to facilitate adaptive evolution, achieving
a formate tolerance of Yarrowia lipolytica to 1 M within two months.
This effort resulted in two mutant strains, designated as M25-70 and
M25-14, both exhibiting significantly enhanced formate utilization
capabilities. Transcriptomic analysis revealed the up-regulation of nine
endogenous genes encoding formate dehydrogenases when cultivated
utilizing formate as the sole carbon source. Furthermore, we uncovered
the pivotal role of the glyoxylate and threonine-based serine pathway in
enhancing glycine supply to promote formate assimilation. The full
potential of Yarrowia lipolytica to tolerate and utilize formate
establishing the foundation for pyruvate carboxylase-based carbon
sequestration pathways. Importantly, this study highlights the existence
of a natural formate metabolic pathway in Yarrowia lipolytica.29 Apr 2024Submitted to Biotechnology Journal 30 Apr 2024Submission Checks Completed
30 Apr 2024Assigned to Editor
30 Apr 2024Reviewer(s) Assigned