Metal ion regulation of Pichia kudriavzevii MZ5 for degradation of
biogenic amines
Abstract
Biogenic amines (BAs) are naturally present in various foods, and high
levels of them have a negative impact on human health. Pichia
kudriavzevii MZ5 exhibits excellent potential in degrading biogenic
amines. Among the tested addition of the culture medium components, Mg2+
is the most effective factor of enhancing the ability of P. kudriavzevii
MZ5 to degrade biogenic amines. After 72 h of cultivation, the total
biogenic amines degradation rate increased from 14.2 % to 43.39 % when
Mg2+presented in the medium. The biomass of P. kudriavzevii MZ5
decreased, but there was no correlation between the yeast biomass and
the biogenic amines degradation rate in this experiment. The addition of
Mg2+ in the medium increased the GSH of P. kudriavzevii MZ5 from 0.34
mg/mg prot to 0.82 mg/mg prot, GST from 2.76 U/mg prot to 3.30 U/mg
prot, and Ca2+- Mg2+- ATPase activity from the original 5.36 U/mg prot
to 17.36 U/mg prot. These results indicate that Mg2+ significate
influenced the degradation ability of P. kudriavzevii MZ5 towards
biogenic amines, which suggest that Mg2+ may have a great potential as a
promoter for microbial degradation of biogenic amines.