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Development of a novel microplate for high-throughput screening and optimization of DHA producing strains based on CFD technology
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  • He Shaojie,
  • Linhui Yang,
  • YuanHang Du,
  • LingLing Tong,
  • Yue Wang,
  • Guo dongsheng
He Shaojie
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Linhui Yang
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YuanHang Du
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LingLing Tong
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Guo dongsheng

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Microtiter plates are suitable for screening and process development of most microorganisms. They are currently the container of choice for high-throughput and small-scale microbial culture, but require optimization for specific work. This research presents a novel type of microtiter plate was developed using computational fluid dynamics (CFD) technology. The new plate provides high oxygen supply and optimal mixing effects for the fermentation culture of docosahexaenoic acid (DHA) producing strains, surpassing the conventional method of strains screening with shake flasks, which is insufficient. the shape of the microtiter plate was modified, and baffles were introduced to improve mass transfer and oxygen supply effects in the vibrating bioreactor. CFD technology was used to model the new plate’s characteristics, establishing the superiority of hexagonal microtiter plates with six baffles. Parameters in the incubation process, such as vibration frequency and liquid load, were optimized, and the final result achieved a KLa of 0.61s-1 and a volume power input of 2364 w/m3, which was 4-5 times better than the original 96-well plate. The culture results optimized by the model were also verified. Therefore, this new microtiter plate provides a powerful tool for future high-throughput screening of strains.
19 Apr 2023Submitted to Biotechnology Journal
19 Apr 2023Submission Checks Completed
19 Apr 2023Assigned to Editor
21 Apr 2023Reviewer(s) Assigned
21 May 2023Review(s) Completed, Editorial Evaluation Pending
23 May 2023Editorial Decision: Revise Major
30 Jun 20231st Revision Received
30 Jun 2023Submission Checks Completed
30 Jun 2023Assigned to Editor
30 Jun 2023Reviewer(s) Assigned
06 Jul 2023Review(s) Completed, Editorial Evaluation Pending
10 Jul 2023Editorial Decision: Revise Major
10 Jul 20232nd Revision Received
11 Jul 2023Submission Checks Completed
11 Jul 2023Assigned to Editor
12 Jul 2023Review(s) Completed, Editorial Evaluation Pending
12 Jul 2023Editorial Decision: Accept