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Performance of a New Family of Modular, Bed-Supported, Chromatography Devices
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  • Gerald Platteau,
  • Guido Stroehlein,
  • James Van Alstine,
  • Masayoshi Nagaya
Gerald Platteau
JSR Life Sciences
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Guido Stroehlein
JSR Life Sciences
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James Van Alstine
JMVA Biotech AB
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Masayoshi Nagaya
JSR Life Sciences

Corresponding Author:[email protected]

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Pre-packed chromatography columns and cassette filtration units offer many advantages in bioprocessing. These include reduced labor costs and processing times, ease of storage, and enhanced process flexibility. Rectangular formats are particularly attractive as they can be stacked and multiplexed together for continuous processing. Cylindrical chromatography beds have historically been favored even though their bed support and pressure-flow performance vary with bed dimensions. This work presents the performance of rectangular chromatography devices with novel internally supported beds. They are compatible with existing chromatography workstations and can be packed with any standard commercial resin. The devices offer pressure-flow characteristics independent of container-volume, simple multiplexing, and separation performance comparable to cylindrical columns. Their internal bed support allows mechanically less-rigid resins to be used at up to 4 times higher maximal linear velocities, and productivities approaching 200 g/L/h for affinity resins, compared to the 20 g/L/h typical of many column-based devices. Three 5 L devices should allow processing of up to 3 kg of monoclonal antibody per hour.
01 Feb 2023Submitted to Biotechnology and Bioengineering
01 Feb 2023Submission Checks Completed
01 Feb 2023Assigned to Editor
01 Feb 2023Review(s) Completed, Editorial Evaluation Pending
05 Mar 2023Reviewer(s) Assigned
13 Apr 2023Editorial Decision: Revise Minor
25 May 20231st Revision Received
25 May 2023Submission Checks Completed
25 May 2023Assigned to Editor
25 May 2023Review(s) Completed, Editorial Evaluation Pending
29 May 2023Editorial Decision: Accept