Background and Purpose: CKD is a clinical syndrome with slow development and gradual deterioration of renal function. At present, modern medicine still lacks an ideal treatment method for this disease, while TCM has accumulated rich clinical experience in the treatment of CKD, which can effectively improve renal function and delay renal failure, and has unique advantages. RC is widely used in clinical practice to treat CKD, especially the ‘Kidney-Yin’ deficiency syndrome. However, the compatibility mechanisms responsible for its effects in experimental studies, including preclinical and clinical researches, are still not fully understood. Experimental Approach: Adenine-induced CKD rats were used to investigated the preventive effect of RC on CKD rats. According to the Illumina-based high-throughput 16S rRNA gene sequencing results, to discuss the flora abundance of rats in different treatment group. Using Chamber, Western blot and PCR were used to investigate the effects of RC on intestinal barrier function and its molecular mechanism in model animals. H&E and M&T staining diagram were used to observe the pathological state of renal tissue in different group. ELISA was used to study the expression of inflammatory factors in different treatment group. Conclusion and Implications: We obtained evidence showing an obvious effect of RC on strengthening the intestinal barrier and modulating gut microbiota in adenine-induced CKD rats. This project revealed the compatibility mechanism of RC in regulating intestinal microecology and barrier function to intervene in CKD, and provided basis and ideas for the clinical application of RC and the development of innovative drugs for CKD.