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A low-cost, portable, and practical LAMP device for point-of-diagnosis in the field
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  • Sumeyra Kaymaz,
  • Ali Ergenç ,
  • Ali Aytekin ,
  • Stuart Lucas,
  • Meltem Elitas
Sumeyra Kaymaz
Sabanci University

Corresponding Author:[email protected]

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Ali Ergenç
Istanbul Technical University
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Ali Aytekin
Yeditepe Universitesi
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Stuart Lucas
Sabanci University
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Meltem Elitas
Sabanci University
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Transition of rapid, ready-to-use, and low-cost nucleic acid-based detection technologies from laboratories to points of sample collection has drastically accelerated. However, most of these approaches are still incapable of diagnosis starting from sampling, through nucleic acid isolation and detection in the field. Here, we developed a simple, portable, low-cost, colorimetric, and remotely controllable platform for reliable, high-throughput, and rapid diagnosis using loop mediated isothermal amplification (LAMP) assays. It consists of a thermally isolated cup, low-cost electronic components, a polydimethylsiloxane sample well, and a fast prototyped case that covers electronic components. The steady-state temperature error of the system is less than 1%. We performed LAMP, Colony-LAMP, and Colony PCR reactions using the yaiO2 primer set for Escherichia coli and Pseudomonas aeruginosa samples at 65˚C and 30 min. We detected the end-point colorimetric readouts by the naked eye under day light. We confirmed the specificity and sensitivity of our approach using pure genomic DNA and crude bacterial colonies. We benchmarked our Colony-LAMP detection against Colony PCR. The number of samples tested can easily be modified for higher throughput in our system. We strongly believe that our platform can greatly contribute rapid and reliable diagnosis in versatile operational environments.
02 Nov 2021Submitted to Biotechnology and Bioengineering
13 Nov 2021Submission Checks Completed
13 Nov 2021Assigned to Editor
15 Nov 2021Reviewer(s) Assigned
09 Dec 2021Review(s) Completed, Editorial Evaluation Pending
09 Dec 2021Editorial Decision: Revise Major
17 Dec 20211st Revision Received
21 Dec 2021Submission Checks Completed
21 Dec 2021Assigned to Editor
22 Dec 2021Reviewer(s) Assigned
22 Dec 2021Review(s) Completed, Editorial Evaluation Pending
22 Dec 2021Editorial Decision: Accept
25 Dec 2021Published in Biotechnology and Bioengineering. 10.1002/bit.28025