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Analysis and Optimal Design of Batch and Two-Column Continuous Chromatographic Frontal Processes for Monoclonal Antibody Purification
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  • Ce Shi,
  • Sebastian Vogg,
  • Dong-Qiang Lin,
  • Mattia Sponchioni,
  • Massimo Morbidelli
Ce Shi
Zhejiang University

Corresponding Author:[email protected]

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Sebastian Vogg
YMC ChromaCon
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Dong-Qiang Lin
Zhejiang University
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Mattia Sponchioni
Politecnico di Milano
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Massimo Morbidelli
Politecnico di Milano
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Frontal chromatography has seen increased interest for protein purification, in particular as a polishing step in downstream processes for therapeutic proteins production, as for example the purification of monoclonal antibodies (mAbs) from high molecular weight impurities, e.g., aggregates, using cation exchange resins. In this work we introduce a new two-column continuous process implementing frontal chromatography. The design procedure and its performance, compared to classical batch technology, are discussed. This represents an additional option in the realisation of optimised continuous downstream processing of therapeutic proteins.
04 Dec 2020Submitted to Biotechnology and Bioengineering
04 Dec 2020Submission Checks Completed
04 Dec 2020Assigned to Editor
10 Dec 2020Reviewer(s) Assigned
04 Jan 2021Review(s) Completed, Editorial Evaluation Pending
04 Jan 2021Editorial Decision: Revise Major
09 Feb 20211st Revision Received
10 Feb 2021Submission Checks Completed
10 Feb 2021Assigned to Editor
19 Feb 2021Reviewer(s) Assigned
12 Mar 2021Review(s) Completed, Editorial Evaluation Pending
12 Mar 2021Editorial Decision: Accept