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Techno-economic analysis of semicontinuous bioreactor production of biopharmaceuticals in transgenic rice cell suspension cultures
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  • Jasmine Corbin,
  • Matthew McNulty,
  • Kantharakorn Macharoen,
  • Karen McDonald,
  • Somen Nandi
Jasmine Corbin
University of California Davis

Corresponding Author:[email protected]

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Matthew McNulty
University of California, Davis
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Kantharakorn Macharoen
University of California Davis
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Karen McDonald
University of California, Davis
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Somen Nandi
University of California, Davis
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Biopharmaceutical protein production using transgenic plant cell bioreactor processes offers advantages over microbial and mammalian cell culture platforms due to the ability to produce complex biologics, use of simple chemically-defined, animal component-free media, robustness of host cells, and biosafety. A disadvantage of plant cells from a traditional batch bioprocessing perspective is their slow growth rate which has motivated us to develop semicontinuous and/or perfusion processes. Although the economic benefits of plant cell culture bioprocesses are often mentioned in the literature, to our knowledge no rigorous techno-economic models or analyses have been published. Here we present techno-economic models in SuperPro Designer® for the large-scale production of recombinant butyrylcholinesterase (BChE), a prophylactic/therapeutic bioscavenger against organophosphate nerve agent poisoning, in inducible transgenic rice cell suspension cultures. The base facility designed to produce 25 kg BChE per year utilizing two-stage semicontinuous bioreactor operation manufactures a single 400 mg dose of BChE for $263. Semicontinuous operation scenarios result in 4-11% reduction over traditional two-stage batch operation scenarios. In addition to providing a simulation tool that will be useful to the plant-made pharmaceutical community, the model also provides a computational framework that can be used for other semicontinuous or batch bioreactor-based processes.
22 Apr 2020Submitted to Biotechnology and Bioengineering
24 Apr 2020Submission Checks Completed
24 Apr 2020Assigned to Editor
27 Apr 2020Reviewer(s) Assigned
15 May 2020Editorial Decision: Revise Major
15 May 2020Review(s) Completed, Editorial Evaluation Pending
06 Jun 20201st Revision Received
06 Jun 2020Submission Checks Completed
06 Jun 2020Assigned to Editor
22 Jun 2020Editorial Decision: Accept
22 Jun 2020Review(s) Completed, Editorial Evaluation Pending