Complete genome and sequence and phylogenetic analyses of the first
bovine norovirus strain identified in northern China
Abstract
Bovine norovirus (BNoV) is a recently identified calicivirus in the
genus Norovirus, which can cause enteric disease with clinical signs
including vomiting, diarrhea, dehydration, and mortality in neonatal
calves. BNoV has been reported in different countries all over the
world. We collected 39 fecal, blood, and tissue samples from calves with
diarrhea from Hebei in northern China in 2019. The presence of BNoV was
determined by PCR only two samples (5.1%) were positive. The complete
genome of the newly identified strain Bo/CH/HB/BD/2019 was successfully
sequenced (GenBank accession number: MN480761). Based on the existing
gene sequences in the GenBank database, evolutionary trees were
constructed. Sequence analysis showed that the nucleotide sequence
homology of the Bo/CH/HB/BD/2019 genome with BNoV genomes in GenBank was
84.0–92.4%. Bo/CH/HB/BD/2019 has a GIII.2 genotype, and further
analysis of its VP1 gene revealed four amino acid substitutions, i.e.,
225C, 246T, 624T, and 945T. To the best of our knowledge, this is the
first time the complete genome of a BNoV isolate from northern China has
been sequenced.