Previous studies have examined the role female presence plays in agonistic displays in the field cricket. 
From these previous studies, we predicted that the frequency agonistic displays would increase between sex-segregated male crickets, regardless of size difference under the presence of female scents. 

Methods

Three 0.283mcircular sheets of filter paper were placed in enclosures of sex-segregated female crickets (n=28; n=16; n=34) over the weekend. The control sheets were placed in a cricket enclosure without any crickets over the weekend.
The sections were on the day of experimentation split cut into 1/12 sections (0.0236m2). Male crickets were selected randomly from male segregated enclosures, and their thoraxes were measured with digital callipers with an error margin of 0.01cm. The measured crickets were then placed in individual tubes to recover for a minimum of one hour. A pair of crickets were then placed into segregated arenas (pictured in figure. 1), with either the control filter paper or experimental filter paper placed in the middle section. The crickets were allowed 5 minutes to acclimatise before the barriers were removed. Levels of cricket aggression were measured using the linearly scaled ethogram from \cite{Stevenson2012} . The crickets were observed for 10 minutes. The experiment and control tests were repeated a total of 36 times. The enclosures were cleaned with isopropyl alcohol to eliminate residual male pheromones from previous experiments.

WELFARE

All treatments of the experimental animals (Gryllus bimaculatus) complied with the Animals (Scientific Procedures) Act 1986, as well as Directive 2010/63/EU on the protection of animals for scientific purposes.