Carbonyl Cyanide m-chlorophenyl hydrazone (CCCP) is a lipid soluble weak acid, and protonophore that selectively increases the bacterial cytoplasmic membranes permeability to H+ ions \cite{Vignais_1972}. It acts by diffusing into the cytoplasmic membrane in its HA form and releasing a H+ ion into the cytoplasm, A- is then free to diffuse through to the positively charged side of the membrane (at a voltage dependant rate) and adsorb another H+ ion \cite{Benz_1983}. As the protons now have an alternative route down their concentration gradient out of the cell, this vastly reduces the effectiveness of the electron transport chain to maintain Δp. As both ΔpH and ΔΨ are reduced under the action of CCCP, the potential ΔG released from returning the [H+] to equilibrium is reduced, making the translocation less energetically favourable, resulting in reduced uptake of [14C]-isoleucine. The results are however, significantly different to the concentration of isoleucine within a cell starved of glucose ( ρ = 0.0009 ), showing how even a damaged ETC is more effective than the inability to power one.