Methods
The precursor (figure 1) was reacted with [11C]methyl iodide in DMF in the presence of DBU at 110ºC for 4 min followed by deprotection with NaOH at 75ºC for 4min. After prep-HPLC purification and solid phase extraction, the product was injected in 8 pigs with and without 1mg/kg non-radioactive MK7246.