Figure 3. The monoclonal GPR-specific response is characterised
by effector signatures.
A. Volcano plot of differentially expressed genes between GPR- and
ATD-specific populations from CL-MCRL. Dashed line indicates p-value
< 0.05. Genes highlighted in red indicate adjusted p-values
corrected by Bonferroni correction < 0.05. B. Volcano plot of
differentially expressed genes between GPR-specific populations from
CL-MCRL and CL-3089 calculated using Mann-Whitney U test. Dashed line
indicates p-value < 0.05. Genes highlighted in red indicate
adjusted p-values corrected by Bonferroni correction < 0.05.
C. Violin plots showing the transcriptional expression level of
effector-associated markers. D. GSEA summary of differentially expressed
genes between GPR- and ATD-specific populations from CL-MCRL, using the
set of Hallmark pathways from the Human MSigDB collections. E. GSEA plot
showing selected enriched pathways between GPR- and ATD-specific
populations from CL-MCRL. NES: normalised enrichment score, pval:
p-value, padj: adjusted p-value. Statistical significance was determined
by Mann-Whitney U test (A and B) and Broad GSEA test (D and E). n.s. not
significant, **** p < 0.0001