Materials and methods
Detailed methods are provided in SI Appendix. Briefly, adult male Jun~Fra-2hep mice were sacrificed at different time point of transgene induction (typically 2 months and 9 months) and the livers harvested for histological, molecular and biochemical analyses. Blood collected by submandibular vein or cardiac puncture was used for longitudinal monitoring. Murine AML12 and human HepG2 liver-derived cell lines were used for in vitroexperiments.