Materials and methods
Detailed methods are provided in SI Appendix. Briefly, adult male
Jun~Fra-2hep mice were sacrificed at
different time point of transgene induction (typically 2 months and 9
months) and the livers harvested for histological, molecular and
biochemical analyses. Blood collected by submandibular vein or cardiac
puncture was used for longitudinal monitoring. Murine AML12 and human
HepG2 liver-derived cell lines were used for in vitroexperiments.