3.5. Cell proliferation activity of
cp-hFGF7115-114
The biological function of hFGF7 can also be proven by the controlling
process for repairing epithelial cells in-vitro ; therefore, we
further tested whether cp-hFGF7115-114 could induce
the proliferation of NIH3T3 cells. A cell proliferation assay for time
course analyses was conducted at 1, 10, and 50 ng/mL
cp-hFGF7115-114 concentrations by using purified
proteins. As shown in Figure 4C, cp-hFGF7115-114retained a cell proliferation activity similar to that of the wild type
hFGF7 under the same concentrations and incubation times. We also
measured the cellular toxicity of cp-hFGF7115-114 by
using NIH3T3 cells. To do so, the cells were treated with
cp-hFGF7115-114 for 24 and 72 h at concentrations of
0.5, 1, 10, 50, and 100 ng/mL. The commercially available hFGF7 was also
used as the control. As a result, a survival rate of 98 to 100% was
shown in the case of the purified cp-hFGF7115-114 when
the cells were treated for 24 h at a concentration of 50 ng/mL. At the
72 h culture time point, the cell viability did not decrease to 95% or
less even with the purified protein at a concentration of 100 ng/mL
(Figure S4). These results confirmed that the purified
cp-hFGF7115-114 did not cause more cytotoxicity than
the wild type, and thus we were unable to observe a distinct difference
between the two proteins.