Figure legends
Figure 1. LL particles can give rise to cell recruitmentin vivo and the calcium InsP6 component does not contribute to the response. Mice were injected i.p. with LL particles containing their intrinsic components mucins and calcium InsP 6 (wpLL) at a dose of 225 µg per mouse or with LL particles containing only their mucin component (pLL) at a dose of 150 µg per mouse (which corresponds to the mass of mucins present in a 225 µg dose of wpLL), or with vehicle only (saline). Total cells and selected cell populations in the peritoneal cavity were quantitated by a combination of cell counter and flow cytometry 24 h later.
Figure 2. The intact mucin glycans are mostly dispensable for inflammation induced by LL particles. Mice were injected i.p. with LL particles containing only their mucin component at a dose of 150 µg per mouse or with vehicle only (saline). The LL particles were untreated (pLL), subjected to a mock treatment (pLL mock) or treated with sodium periodate for oxidation of terminal monosaccharide residues (pLL periodate). Total cells and selected cell populations in the peritoneal cavity were quantitated by a combination of cell counter and flow cytometry 24 h later.