Figure legends
Figure 1. LL particles can give rise to cell recruitmentin vivo and the calcium InsP6 component
does not contribute to the response. Mice were injected i.p. with LL
particles containing their intrinsic components mucins and calcium
InsP 6 (wpLL) at a dose of 225 µg per mouse or
with LL particles containing only their mucin component (pLL) at a dose
of 150 µg per mouse (which corresponds to the mass of mucins present in
a 225 µg dose of wpLL), or with vehicle only (saline). Total cells and
selected cell populations in the peritoneal cavity were quantitated by a
combination of cell counter and flow cytometry 24 h later.
Figure 2. The intact mucin glycans are mostly dispensable for
inflammation induced by LL particles. Mice were injected i.p. with LL
particles containing only their mucin component at a dose of 150 µg per
mouse or with vehicle only (saline). The LL particles were untreated
(pLL), subjected to a mock treatment (pLL mock) or treated with sodium
periodate for oxidation of terminal monosaccharide residues (pLL
periodate). Total cells and selected cell populations in the peritoneal
cavity were quantitated by a combination of cell counter and flow
cytometry 24 h later.