Figure
2. FrAdV1 Pre-pVII proteins localizes to the cell nucleus, and
accumulates in the nucleoli. (A) Schematic representation of the
GFP-fusion proteins expressed. Proteins are represented by white
horizontal bars . NLSs are shown as blue vertical bars , along
with their amino acidic sequence. The single letter amino acid
code is used. Basic residues are in boldface . (B) The indicated
GFP fusion proteins were transiently co-expressed in HEK293A with
DsRed-fibrillarin by means of Lipofectamine 2000 transfection. 24h post
transfection, cells were incubated with DRAQ5 to stain cell nuclei,
fixed with paraformaldehyde and processed for CLSM analysis as described
in the Materials and Methods section. Representative images of the 633
nm (nuclei), 488 nm (GFP), and 568 (fibrillarin) laser channels are
shown, along with a merged image (merge), and a rgb profile plot across
the indicated area.