Figure 2. FrAdV1 Pre-pVII proteins localizes to the cell nucleus, and accumulates in the nucleoli. (A) Schematic representation of the GFP-fusion proteins expressed. Proteins are represented by white horizontal bars . NLSs are shown as blue vertical bars , along with their amino acidic sequence. The single letter amino acid code is used. Basic residues are in boldface . (B) The indicated GFP fusion proteins were transiently co-expressed in HEK293A with DsRed-fibrillarin by means of Lipofectamine 2000 transfection. 24h post transfection, cells were incubated with DRAQ5 to stain cell nuclei, fixed with paraformaldehyde and processed for CLSM analysis as described in the Materials and Methods section. Representative images of the 633 nm (nuclei), 488 nm (GFP), and 568 (fibrillarin) laser channels are shown, along with a merged image (merge), and a rgb profile plot across the indicated area.