Synopsis:
The study explores the nuclear transport mechanism of the precursor
protein VII from an ancient siadenovirus that utilizes multiple nuclear
localization signals (NLSs) and pathways for nuclear delivery. It
identifies two strong NLSs that are key drivers of this process,
interacting with specific importins. Additionally, a weaker NLS and
another signal are responsible for targeting of the nucleolus. The study
reveals that the import process is different between the frog and human
virus counterparts, rely on different pathways, and indicates that
adenoviruses have evolved diverse strategies to ensure the nuclear
import of pVII proteins. These findings offer insights into the
evolutionary aspects of adenovirus interactions with host nuclear
transport systems.