Figure 1. The evolution of pVII in siadenoviruses and identified NLSs in FrAdV1 pVII. (A ) The phylogenetic tree exhibits the possible evolutionary relationship of siadenoviruses based on protein VII. The numbers on the left show bootstrap values as percentages, and the labels at branch tips refer to adenovirus species name, followed by GenBank accession number in parentheses. (B ) Frog adenovirus 1 (FrAdV1 pVII) (GenBank Accession no: NC_002501) 7 and human adenovirus (HAdV pVII) (GenBank Accession no: AC_000008) sequences were aligned using Geneious Prime (version 21.1.1, Biomatters, Ltd., Auckland, New Zealand) and previously described NLSs and NoLS are highlighted in dark and light blue shadings respectively. (C ) Simplified diagram displaying the identified putative NLS regions of FAdV pVII and previously described NLSs and NoLS from HAdV27,8,10.