Fig.S1: Differential gene expression and pathway analysis in MHV-1 vs mock-infected lean and obese C3H/HeJ mice.
RNAseq was performed on the RNA collected from blood of MHV-1 and mock-infected lean and obese C3H/HeJ mice at 2 dpi. (A & B) Volcano plot of differentially expressed genes (DEGs) for lean (A) and obese (B) mice. For identification of DEGs, genes with p-adjusted values < 0.1 and log2FoldChange > 1, were selected by integrated Differential Expression and Pathway (iDEP .96) analysis tool. (C & D) Pathway analysis for lean (C) and obese (D) mice. Pathways were generated using Gene Set Enrichment Analysis (GSEA) using the Kyoto Encyclopedia of Genes and Genomes (KEGG). All the pathway analysis data were compared to data from the blood of COVID-19 patients with severe disease; orange arrows indicate common upregulated and blue arrows indicate common downregulated pathways between MHV-1 infected vs mock and severe vs mild COVID-19 patients and the common up or downregulated pathways are represented by arrows.