EGCG attenuate the expression levels of Notch in colitis mice
It have been confirmed that Notch is a new target of EGCG17. In order to further understand the mechanism of EGCG improved the colitis, the expression levels of protein which is belong to Notch signaling pathway were detect by WB in colon from colitis mice. The expression levels of Notch1 and Notch2 were increase in colitis mice colon compared with normal group (figure5A, 5B and 5C). And the phenomenon is alleviated by EGCG treatment (figure5A, 5B and 5C). The Notch1 was activated in DSS group and deactivated with EGCG (figure5A and 5D). The expression levels of Notch downstream target protein HES-1 were also decreased in EGCG group compared with DSS group. The same results were observed in the colon stained by IHC of Notch1 and HES-1 (figure 5F). These results indicated that EGCG attenuates colitis in mice through the Notch pathway.