Figure 1 EGCG could attenuate inflammatory responses by targeting the Notch pathway in Caco-2 cells. The supernatant and samples were obtained from the Caco-2 cell line after LPS stimulation with or without EGCG treatment for 24 h. To measure the pro-inflammatory marker TNF-α, the supernatant was treated as described earlier using ELISA. 60 μg of each sample was applied to each well for detection of inflammation protein and Notch signaling pathway protein. (A ) TNF-α was detected to demonstrate whether EGCG attenuates inflammatory response in Caco-2 cells. (B, C ) The protein expression of COX2 was measured by western blotting, and the ODs were determined. (D ) The protein levels of Notch1, Notch2, Cleaved-Notch1, and Hes-1 were detected by western blotting, and the ODs were calculated to demonstrate the effect of EGCG on the Notch signaling pathway in the Caco-2 cell. Data are pooled from two experiments. Data are presented as the means ± SEM. *P<0.05, **P<0.01, ***P<0.001 (one-way ANOVA). EGCG, (-)-epigallocatechin gallate; LPS, Lipopolysaccharide.