Figure 4 Macrophages were decreased by treat with EGCG in colon
of the UC mice. Immunohistochemisty was performed on paraffin sections
stained for F4/80 and
iNOS(A). The Brown area (red arrows) indicates positive staining. The
tissue was obtained from the colon of mice was homogenized in protein
extraction solution and 60 μg for each well for detected. The protein
expressions of iNOS (B) were measured by western blotting. The value of
OD (C) was detected by Image J (NIH). Data are pooled from two
experiments (n=6 colon from six mice per group, repeated twice). Data
are presented as the means ± SEM. *P<0.05, **P<0.01,
***P<0.001 (one-way ANOVA, EGCG, and normal group vs. DSS
group). Scale bars, 100 μm. LE, Low dose of (-)-epigallocatechin
gallate; HE, High dose of (-)-epigallocatechin gallate; DSS, Dextran
sulfate sodium.