EGCG attenuate the expression levels of Notch in colitis mice
It have been confirmed that Notch is a new target of
EGCG17. In order to further understand the mechanism
of EGCG improved the colitis, the expression levels of protein which is
belong to Notch signaling pathway were detect by WB in colon from
colitis mice. The expression levels of Notch1 and Notch2 were increase
in colitis mice colon compared with normal group (figure5A, 5B and 5C).
And the phenomenon is alleviated by EGCG treatment (figure5A, 5B and
5C). The Notch1 was activated in DSS group and deactivated with EGCG
(figure5A and 5D). The expression levels of Notch downstream target
protein HES-1 were also decreased in EGCG group compared with DSS group.
The same results were observed in the colon stained by IHC of Notch1 and
HES-1 (figure 5F). These results indicated that EGCG attenuates colitis
in mice through the Notch pathway.