Test-retest Reliability at Network Level
To examine whether and how the reliability estimates of ANT varied among
intrinsically organized networks, we summarized the region-level ICC
estimates by the seven networks defined by the Schefer-Yeo atlas
(Figure 4 , supplementary Table S6 ).
We used a linear mixed-effect model to examine whether the seven
networks have differential reliability in alerting, orienting, and
executive networks. There was a significant interaction between the
intrinsic and attention network contrast
(F (12, 386) = 2.327,p = 0.007). Simple effect analysis revealed that the
frontoparietal, default mode, and limbic network showed differential
reliability for the three attention network contrasts
(supplementary Table
S7 ). The frontoparietal network exhibited higher ICC on the
orienting contrast than the executive contrast (t = 2.449,
p = 0.039) and alerting contrast (t386 =
2.557, p = 0.029). The default network showed inferior
reliability on the executive contrast compared with the alerting
(t386 = -2.839, p = 0.013) and orienting
contrast (t386 = -2.687, p = 0.021).
In addition, we also examined the interaction between the ANT condition
(congruent, incongruent, center cue, spatial cue, no cue) and the seven
intrinsic networks (F (24, 772) = 7.129, p < 0.001).
Congruent and incongruent conditions showed higher ICC than the other
conditions in all intrinsic networks except for the limbic network
(supplementary Table S8) . At least half of the regions in
dorsal attention, ventral attention, visual attention, and somatomotor
network can have acceptable reliability for the congruent and
incongruent conditions. Finally, comprehensive pairwise
comparisons among the eight contrasts supported that ICC of congruent
and incongruent conditions were higher than all other contrasts for all
intrinsic networks except for the limbic network (supplementaryTable S9). The reliability of the estimates also corresponded
well to the effect size (BOLD percent signal change) estimates at the
intrinsic network level (Figure S5) .