Calculations of C, N and P pools
At each site, the biomass of different plant components (leaves, woody
tissues and roots for both overstory trees, and understory trees, shrubs
and grasses) were estimated either using the whole-harvest-method or
locally developed allometric equations that relates biomass to field
measured tree height and diameter at the breast height (see Text S2 of
Tang et al. (2018) ). C, N, and P concentrations (in unit of g C
or N or P per g dry matter) of different plant components were measured
in laboratory using the biomass samples taken at the sites and used to
calculate C, N and P pools in unit of g C or N or P
m-2 at each site (Equation 1). C, N and P pools of the
surface litter were estimated from the measured elemental concentrations
and the dry weight of litter (Equation 2). For soil, soil core samples
were taken from five different depth intervals (0-10cm, 10-20cm,
20-30cm, 30-50cm, 50-100cm). Bulk density, gravel content, and total C,
N, and P concentration were measured at each depth to calculate soil C,
N and P pools (Equation 3). That is
\(X_{B}={\sum_{i=1}^{3}B_{i}\times x}_{i},\ \ i=1,\ 2,\ 3\) (1)
\(X_{L}=L\times x_{L}\) (2)
\(X_{S}=\ \sum_{j=1}^{5}{\left(1-g_{i}\right)\times\rho_{i}}\times x_{j}\times d_{j}\times 10\)(3)
where B i is the biomass of component i(leaf, wood or root) in g (dry matter) m-2, andx i is C, N or P concentration in biomass
component i in g C or N or P g-1 (dry matter).L is dry weight of litter in g (dry matter)
m-2, and x L is C, N or P
concentration of litter biomass in g C or N or P g-1(dry matter). gj represents the volume percentage
of gravels with a diameter >2 mm in soil layer j ,ρj is bulk density (g cm-3),xj is total C, N or P content of soil in g C or N
or P per kg soil, and dj is the thickness in cm
of the j th layer. A factor 10 is for converting
from g 10-3cm-2 to g
m-2.