Figure 4. Effect of eperisone on bleomycin-induced increases in myofibroblasts.
Mice were treated with bleomycin (BLM, 1 mg/kg) or vehicle once only on day 0. The mice were then orally administered the indicated dose of eperisone (Epe) once daily for 9 days (from day 10 to day 18). Pulmonary tissue sections were prepared on day 20 and subjected to immunohistochemical analysis with an antibody against α-smooth muscle actin (SMA) (scale bar = 100 µm) (A). The α-SMA-positive area was determined using ImageJ software (B). Values represent the mean ± SEM **P < 0.01 ; *P < 0.05.