Figure 1. Preferential suppression of fibroblast activity by
eperisone.
LL29 or A549 cells were incubated with the indicated concentrations (µM)
of eperisone for 24 h. The percentage of viable cells was determined
using a CellTiter-Glo® 2.0 assay (A). LL29 cells were incubated with the
indicated concentrations (µM) of eperisone for 18 h. The cytotoxicity
was measured every hour using CellTox™ Green Dye and a microplate reader
(excitation: 485 nm, emission: 530 nm) (B). LL29 cells were incubated
with transforming growth factor (TGF)-β1 (5 ng/ml) for 72 h in the
presence of the indicated concentrations of eperisone. Total RNA was
extracted and subjected to real-time RT-PCR using a specific primer set
for each gene. The values were normalized to Gapdh gene
expression and expressed relative to the control sample (C). Values
represent the mean ± SEM
** P <
0.01 ; *P < 0.05 ; NS, not significant.