Colonic segments were immersed in paraffin wax and stained with
haematoxylin and eosin (H&E). For histopathological evaluation of
colitis, each segment was evaluated as regards: architectural
distortion, inflammatory infiltration by (neutrophils, eosinophils,
lymphocytes and macrophages), the incidence of cryptitis and mucosal
ulceration. Each parameter was then scored in a range of 0-3, (0=
unaffected, 1= mild, 2= moderate and 3= severe).