2.4. Histopathological analysis
Colonic segments were immersed in paraffin wax and stained with haematoxylin and eosin (H&E). For histopathological evaluation of colitis, each segment was evaluated as regards: architectural distortion, inflammatory infiltration by (neutrophils, eosinophils, lymphocytes and macrophages), the incidence of cryptitis and mucosal ulceration. Each parameter was then scored in a range of 0-3, (0= unaffected, 1= mild, 2= moderate and 3= severe).