Subjects.
we collected 14 products of conception in the present study, from the
First Affiliated Hospital of Zhengzhou University (Zhengzhou China),who
were normal for karyotype and chromosome microarray analysis between
October 2020 and February 2021.Among the 14 cases, 7 fetuses were
diagnosed as congenital developmental abnormalities by ultrasound, and
the parents chose to terminate the pregnancy. The remaining 7 fetuses
were intrauterine stillbirths of unknown causes. The time of fetal death
or termination of pregnancy occurs between 8 and 26 weeks, and the
average gestational age is about 18 weeks. Six cases were less than 12
weeks old. All the couples were not first Cousins. Through inquiring the
medical history and inquiring the relevant clinical examination results,
it can be found that none of the 14 cases had a clear family history of
genetic diseases. The pregnant women themselves have no uterine
abnormalities, hypertension, diabetes and other basic diseases, the age
of 25-34 years old. The villus tissue, fetal skin tissue and the
peripheral blood of the husband and wife were extracted from the fluid
products and tested by Trio-WES. The present study was approved by the
ethical committee of the First Affiliated Hospital of Zhengzhou
University. Written informed consent was obtained from each participant.