Experiment locations and climate
Trans-Mediterranean translocation of Posidonia oceanica fragments
took place between Catalunya (Spain), Mallorca (Spain) and Cyprus in
July 2018 and were monitored until July 2019 (Fig. 1). Sea surface
temperature data for each transplant site were based on daily SST maps
with a spatial resolution of 1/4°, obtained from the National Center for
Environmental Information (NCEI,https://www.ncdc.noaa.gov/oisst (Reynolds et al.2007). These maps have been generated through the optimal interpolation
of Advanced Very High Resolution Radiometer (AVHRR) data for the period
1981-2019. Underwater temperature loggers (ONSET Hobo pro v2 Data
logger) were deployed at the transplant sites in Catalunya, Mallorca and
Cyprus and recorded hourly temperatures throughout the duration of the
experiment (one year). In order to obtain an extended time series of
temperature at transplant sites, a calibration procedure was performed
comparing logger data with sea surface temperature from the nearest
point on SST maps. In particular, SST data were linearly fitted to
logger data for the common period. Then, the calibration coefficients
were applied to the whole SST time series to obtain corrected-SST data
and reconstruct daily habitat temperatures from 1981-2019. Local climate
data was also compared to the global thermal distribution of P.
oceanica to assess how representative experimental sites were of the
thermal distribution of the species (Supplementary materials).
Collectively, seawater temperatures from the three locations span the
16th - 99th percentile of
temperatures observed across the global thermal distribution of P.
oceanica. As such Catalunya, Mallorca and Cyprus are herein considered
to represent the cool-edge, centre and warm-edge of P. oceanicadistribution, respectively.
Transplantation took place toward warmer climates and procedural
controls were conducted within each source location, resulting in six
source-to-recipient treatments (Fig. 1). Initial collection of P.
oceanica , handling and transplantation was carried out simultaneously
by coordinated teams in July 2018 (Table S1). Each recipient location
was subsequently resampled four times throughout the experiment, in
August/September 2018 (T1), October 2018 (T2), April 2019 (T3) and
May/June 2019 (T4, Table S1).
Between 60-100 fragments were collected for each treatment. A fragment
was defined as a section of P. oceanica containing one apical
shoot connected with approximately five vertical shoots and 10-15 cm of
rhizome with intact roots. Collection occurred at two sites within each
location, separated by approximately 1 km. Within sites, collections
were conducted between 4 – 5 m depth and were spaced across the meadow
to minimise the dominance of a single clone and damage to the meadow.
Upon collection, fragments were transported for up to one hour back to
the nearest laboratory in shaded seawater.