Study sites and samplingÂ
Three pairs of great tit populations in urban and forest environments
were sampled in the three European cities of Barcelona (Spain),
Montpellier (France) and Warsaw (Poland), Figure 1. For each location,
10 individuals were sampled within the city and 10 individuals were
sampled from nearby forest. Blood samples were collected from breeding
individuals during spring between 2016 and 2018 (except 2 individuals
for Barcelona city, collected in 2014 and 2015) and kept in 96% Ethanol
or Queen’s Lysis Buffer. Samples had balanced sex ratio (5 males and 5
females for each population) except for the forest population of
Barcelona where 6 females and 4 males were sampled.