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\begin{document}

\title{Commercialized kits to assess T-cell responses against SARS-CoV-2 S
peptides}



\author[1]{Monica Martinez Gallo}%
\author[1]{Juliana Esperalba-Esquerra}%
\author[1]{Ricardo Pujol-Borrell}%
\author[1]{Victor Sanda}%
\author[1]{Iria Arrese-Muñoz}%
\author[2]{Candela Fernandez-Naval}%
\author[1]{Andres Anton-Pagarolas}%
\author[3]{Victoria Cardona}%
\author[1]{Moises Labrador-Horrillo}%
\author[1]{Tomas Pumarola-Suñe}%
\author[1]{Manuel Hernandez-Gonzalez}%
\affil[1]{Hospital Universitari Vall d'Hebron}%
\affil[2]{VHIR}%
\affil[3]{Hospital Vall d'Hebron}%


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  \date{June 14, 2024}


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\begin{abstract}
Background: There is an urgent need to assess the levels of protection
generated by natural infection or SARS-CoV-2 vaccines, especially in
individuals professionally exposed. Measuring T-cell responses may
complement antibody tests currently in use as correlates of protection.
Our aim was to assess the feasibility of a validated assay of T-cell
responses applicable to large number of samples. Methods: Twenty
health-care-workers (HCW) were included. Antibody test to SARS-CoV-2 N
and S-proteins in parallel with a commercially available
whole-blood-interferon-gamma-release-assay (IGRA) to S-peptides and two
detection methods, CLIA and ELISA were determined. Results: IGRA test
detected T-cell responses in naturally exposed and vaccinated HCW
already after first vaccination dose. The correlation by the two
detection methods was very high (R\textgreater{}0.9) and sensitivity and
specificity ranged between 100 and 86\% and 100-73\% respectively. Even
though there was a very high concordance between antibody and the IGRA
assay in the ability to detect immune response to SARS-CoV-2, there was
a relatively low quantitative correlation. In the small group primed by
natural infection, one vaccine dose was sufficient to reach immune
response plateau. IGRA was positive in one, with Ig(S) antibody negative
vaccinated immunosuppressed HCW illustrating another advantage of the
IGRA-test. Conclusion: Whole-blood-IGRA-tests amenable to automation and
constitutes a promising additional tool for measuring the state of the
immune response to SARS-CoV-2; they are applicable to large number of
samples and may become a valuable correlate of protection to COVID-19,
particularly for%
\end{abstract}%



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\verb`Manuscript_Allergy with Patient consent statement.doc`  available at \url{https://authorea.com/users/728416/articles/709541-commercialized-kits-to-assess-t-cell-responses-against-sars-cov-2-s-peptides}

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