Experimental Groups and Protocol
Experimental Groups and Protocol were formed in three groups. Group 1
(Sham, n = 5): During the study, no procedure was performed except for
anesthesia. Tissue and blood samples were taken at an hour corresponding
to the end of the reperfusion period. Group 2 (Control, I / R, n = 5):
After anesthesia, 60 minutes of ischemia and 60 minutes of reperfusion
were applied to the infrarenal abdominal aorta, no medication was given.
Tissue and blood samples were taken at an hour corresponding to the end
of the reperfusion period. Group 3 (I / R + Curcumin, n = 5): 60 minutes
of ischemia and 60 minutes of reperfusion were applied to the infrarenal
abdominal aorta after anesthesia. 200 mg/kg Curcumin was administered
intraperitoneally 5 minutes before the tourniquet was opened. (It has
been shown that curcumin improves oxidative stress and inhibits the
expression of inflammatory cytokines, and 200mg / kg as a dose is more
effective 17. After comparing the curcumin doses used
in ischemia reperfusion models, the curcumin dose was applied as
200mg/kg in our study). Tissue and blood samples were taken in the
period corresponding to the end of the reperfusion period.