Total Antioxidant Capacity (TAC)
TAC level of the samples was measured using Rel Assay brand commercial kits. The measurement method was applied on the basis that all antioxidant molecules in the sample reduce the colored ABTS cationic radical and decolorize the colored radical in proportion to the total concentrations of the antioxidant molecules. Trolox, a water-soluble analog of vitamin E, was used as the calibrator. Results were expressed in mmol Trolox Equivalent / L 18. Micromolar (μmol) changed when calculating trolox equivalent per liter.