Ischemia-Reperfusion Injury Model
Ketamine 87 mg/kg intraperitoneally (Ketalar; Parke Davis, Eczacibasi,
Istanbul, Turkey) and Xylazine 13 mg/kg (Rompun; Bayer AG, Leverkusen,
Germany) were administered to all rats used in the experiment after 8
hours of fasting. When necessary, an additional dose was planned for
once during the experiment. Midline laparotomy was performed on rats
whose skin was prepared aseptically. After removing the intestines with
wet gauze, the infrarenal abdominal aorta (IAA) was carefully explored.
The IAA was put under a non-traumatic microvascular clamp. The
microvascular clamp on the IAA was removed after 60 minutes, and 60
minutes of reperfusion was achieved. Aortic ischemia; aortic reperfusion
with loss of pulsation in the distal aorta during the clamping
procedure; confirmed by testing the restoration of pulsation in the
distal aorta after clamp removal. In the control group, laparotomy and
abdominal aortic dissection were performed in equal time (I / R time =
120 minutes), but I / R was not created in this group. To minimize the
loss of heat and fluid from the peritoneal cavity during I / R periods;
after the clamps were placed in the IAA and removed, saline was applied
to the peritoneal cavity and the abdominal incision was temporarily
covered with wet gauze. At the end of the reperfusion period, in all
rats, the median laparotomy incision was opened in the mediastinum by
moving upwards, the heart was reached, and blood was taken from the
right ventricular cavity with the help of a 5-cc syringe. Subsequently,
a right gastrocnemius abdominal aorta sample was taken. Abdominal aortic
samples were stored in 10% formaldehyde solution until evaluation of
immunohistochemical and hematoxylin-eosin. Blood taken from rats was
centrifuged at 4000 rpm for 10 minutes and rat plasma samples were
stored at -20 degrees until biochemical analysis.