Histopathological Examination of Vascular Endothelial Structure
For histopathological examination, aortic tissues were separately fixed in 10% buffered neutral formaldehyde solution. The samples were embedded in paraffin blocks. The 5-micron meter parts were taken. It was stained with hematoxylin eosin dye. A magnification of 20 objectives was used. (Olympus BX51 TF, USA) Interstitial edema, inflammatory cell infiltration and necrosis were evaluated as histopathological parameters. Ranking scores were organized and reported (No: 0, Yes: 1, Significant: 2) for each histopathological parameter. By summing all parameter scores for each sample, the histological score was calculated.