5. The main steps of vaccine design
Vaccine design generally involves going through three stages of appropriate antigen prediction, vaccine platform determination and the suitable vaccination route along with effective regimen [8]. The immunostimulatory strength of the viral antigen, necessity for adjuvant and the nature of the primed protective immune responses depends primarily on vaccine platform. These features also influence the competency of a vaccine candidate for a special route of administration and the need for a booster dose in order to establishing a durable protective immune response. Moreover, certain types of vaccines such as live attenuated or mucosal vaccination should be presented with more pedant safety analysis.