Preparation of soil sample to determining soil elements and analysis
Five grams of soil was dried (80 °C) in a hot air oven and then ground. Carbon concentration was analyzed in a CHN elemental analyzer (Perkin Elmer, 2400 Series II). For measuring pH, 5 g of soil was mixed with 12.5 ml autoclaved distilled water (1:2.5 ratio) and allowed to equilibrate for 30 mins before measurement on a pH meter.