Analysis of repeated measurements
Linear Mixed Effects model: A Linear mixed effect model was built with
sHLA-G as a function of gestational age with the group membership
(SGA/AGA) as a fixed effect and participant ID as a random effect using
lme4 package under the R statistical language and environment
(www.r-project.org). The equation used was as follows: sHLA-G
~ time (in days) + Group + (time (in days)
|Participant ID). Another model devoid of group membership
(SGA/AGA) was constructed. The equation used was as follows: sHLA-G
~ time (in days) + (time (in days) |Participant
ID). Both the models were compared using ANOVA function to evaluate
whether being in SGA or AGA group was associated with variations in
sHLA-G trajectories across pregnancy.