Regulatory mechanism of hsa_circ_0072697 in AS-PBMCs
To investigate the regulatory mechanism of hsa_circ_0072697 in PBMCs
of AS patients, we performed overexpression and knockdown of
hsa_circ_0072697 in PBMCs, and divided them into 5 groups: AS-PBMC,
AS-PBMC+pcDNA3.1 hsa_circ_0072697-NC, AS-PBMC+pcDNA3.1
hsa_circ_0072697, AS-PBMC+siRNA-hsa_circ_0072697-NC,
AS-PBMC+siRNA-hsa_circ_0072697. CCK-8 assay showed that the cell
proliferation activity of the hsa_circ_0072697 overexpression group
was significantly reduced compared to the other four groups, while the
cell proliferation activity of the siRNA-hsa_circ_0072697 group was
increased (Fig. 9A). These results suggest that hsa_circ_0072697 may
play an inhibitory role in the pathogenesis of AS. Furthermore,
apoptosis analysis by flow cytometry showed that the hsa_circ_0072697
overexpression group had the highest apoptosis rate, while the
siRNA-hsa_circ_0072697 group had the lowest apoptosis rate (Fig. 9B).
This indicates that hsa_circ_0072697 is also involved in regulating
the apoptosis of PBMCs. Finally, from cell cycle analysis performed by
flow cytometry, we observed that the hsa_circ_0072697 overexpression
group had a decrease in the number of cells in the G1 phase and an
increase in the number of cells in the S and G2 phases compared to the
control group, while the siRNA-hsa_circ_0072697 group had completely
opposite effects on the cell cycle of PBMCs
(Fig. 9C). These findings indicate
that hsa_circ_0072697 plays an important role in the cell cycle of
PBMCs.