Cell proliferation assay
Cells were seeded into 96-well plates at a density of
5×103 cells per well and incubated at 37°C in a
humidified atmosphere containing 5% CO2. After 24 hours
of incubation, the cells were treated with different concentrations of
test compounds for various time periods. At the end of the treatment,
the medium was removed, and 100 μL of fresh medium containing 10 μL of
CCK-8 solution (BA00208, BIOSS) was added to each well. The plates were
then incubated for an additional 2 hours at 37°C. The absorbance was
measured at 450 nm using a microplate reader (BioTek, USA).