hsa_circ_0072697 regulates NF-kB pathway activation by
modulating p-NF-κB expression in PBMCs of AS patient
In addition, we performed IF assays to investigate the expression of
p-NF-κB and p-IKBα in PBMCs. As shown in
Figure 12A, there was more green
fluorescence in the AS-PBMC+pcDNA3.1 hsa_circ_0072697 group,
indicating positive expression of p-IKBα in this group. However, Figure
12B showed that p-NF-κB was positively expressed (with a higher amount
of green fluorescence) in the AS-PBMC+siRNA-hsa_circ_0072697 group.
These results suggest that hsa_circ_0072697 may regulate NF-κB pathway
activation by modulating p-NF-κB expression.