Impact of hsa_circ_0072697 expression levels on inflammatory
cytokine concentrations in PBMCs of AS patients
Inflammatory cytokines, such as TNF-α, IL-4, IL-10, and IL-17, are
involved in the pathogenesis of many inflammatory diseases, including
AS(37-39). In this study, we investigated the effect of different
expression levels of hsa_circ_0072697 on the concentration of
inflammatory cytokines in PBMCs from AS patients using ELISA assay. As
shown in Figure 10, the concentration of IL-4 and IL-10 was highest in
the AS-PBMC+pcDNA3.1 hsa_circ_0072697 group, while the concentration
of IL-17 and TNF-α was the lowest. In contrast, with the knockdown of
hsa_circ_0072697 expression, the concentration of IL-17 and TNF-α
increased, while the concentration of IL-4 and IL-10 significantly
decreased. These results suggest that hsa_circ_0072697 may regulate
the expression of inflammatory cytokines in AS, and targeting
hsa_circ_0072697 may be a potential therapeutic strategy for AS
treatment.