RT-qPCR-based validation of RNA-seq results
To verify the validity of the RNA-seq data generated above, six DECs
were selected for RT-qPCR-based validation in a larger cohort of PBMC
samples. The observed patterns of differential circRNA expression in AS
and control samples were confirmed by these analyses. Specifically,
hsa_circ_0000702, hsa_circ_0006209, and hsa_circ_0047920 were
found to be considerably upregulated, while hsa_circ_0001543,
hsa_circ_0072697, and hsa_circ_0005076 were significantly
downregulated (Fig. 4). These RNA-seq results were deemed stable and
reliable. As such, these RNA-seq
results were deemed stable and reliable.