Impact of hsa_circ_0072697 expression levels on inflammatory cytokine concentrations in PBMCs of AS patients
Inflammatory cytokines, such as TNF-α, IL-4, IL-10, and IL-17, are involved in the pathogenesis of many inflammatory diseases, including AS(37-39). In this study, we investigated the effect of different expression levels of hsa_circ_0072697 on the concentration of inflammatory cytokines in PBMCs from AS patients using ELISA assay. As shown in Figure 10, the concentration of IL-4 and IL-10 was highest in the AS-PBMC+pcDNA3.1 hsa_circ_0072697 group, while the concentration of IL-17 and TNF-α was the lowest. In contrast, with the knockdown of hsa_circ_0072697 expression, the concentration of IL-17 and TNF-α increased, while the concentration of IL-4 and IL-10 significantly decreased. These results suggest that hsa_circ_0072697 may regulate the expression of inflammatory cytokines in AS, and targeting hsa_circ_0072697 may be a potential therapeutic strategy for AS treatment.