3.4 The effect of MPO in inflammatory response of C. rodentium-infected mice under hypoxia.
Compared with the control mice (Figure 5a-d), the infected mice had significantly increased expression levels of the inflammatory factors TNF-α, IFN-γ and IL-1β in the serum, spleen and colon, and the IL-1β levels in the serum were also significantly increased. The expression of IL-6 was significantly increased in the serum ( P<0.05), while the expression of the above factors decreased under hypoxia exposure, which suggests that the immune response was downregulated under hypoxia (P <0.05). Comparing WT mice and MPO-/- mice, the content of TNF-α in the KI and HKI groups was different from that in the NI and HI groups. Comparing the IL-1β, IFN-γ and IL-6 levels between the KI and HKI groups with those in the NI and HI groups, there was no significant difference, which indicated that the MPO gene has no effect on the production of inflammatory factors in the body.