2.8 Statistical analysis
Statistical analysis was performed using SPSS 27.0 software. The measurement data are expressed as \(\overset{\overline{}}{x}\ \)± S , and multigroup comparisons were undertaken by single-factor variance analysis or nonparametric statistics (rank sum test). The validation test was performed by the Levene test. When the variance was quarriable, the SNK test was used, and the multigroup comparison was chosen when it was different. P <0.05 indicated that the difference was statistically significant.