Figure captions
Figure 1 . Changes in the body weight of mice in each group (mean±SD, n= 8/group; & 0.05 NI vs. NC; @P<0.05 KI vs. KC; ¥P<0.05 HKI vs. HKC; +P<0.05 KI vs. NI; #P<0.05 HI vs. HC; *P<0.05 HKI vs. HI).
Figure 2 . a: Twenty-four-hour results of mouse faecal smear culture. C. rodentium grew well on MacConkey medium.
b: The number of bacteria excreted by mice in each group (mean ± SD, n= 8/group; *P˂0.05 vs. NI; #P˂ 0.05 vs. KC; +P˂ 0.05 vs. KI; *P˂ 0.05 vs. HKI).
Figure 3 . a: Photographs of the colons of mice in each group. Compared with that in the normal group, the colon morphology of the mice in the infected group changed to varying degrees; the main symptoms were shortening, and individual mice had colon thinning and ulceration.
b: Changes in the colon length of mice in each group (mean±SD, n= 8/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC;+P<0.05 vs. KI; 0.05 vs. NC;¤ 0.05 vs. HC; #P<0.05 vs. NI; *P<0.05 vs. HI).
Figure 4 . a: Pathological sections of the colons of mice in each group. According to the comparison, the intestinal mucosal tissue damage in the HI and KI groups was more serious, and the main symptoms were thinning of the intestinal wall, becoming almost transparent, with more severe oedema, disordered villi with different lengths, and not only shallower but also a reduced number of crypts. These alterations were accompanied by a large amount of inflammatory cell infiltration.(20×)
b: Results of pathological scoring of mice in each group. Compared with those of the NI and HI groups, the pathological results of the infection group were more significant with hypoxia (P < 0.05), indicating that hypoxia can aggravate the mouse pathological lesions of the colon (mean±SD, n= 8/group; & 0.05 vs. NC; ▪ P<0.05 vs. HC; *P<0.05 vs. NI; @P <0.05 vs. KC; ¥P<0.05 vs. HKC;+P<0.05 vs. KI; 0.05 vs. NC; ¤ 0.05 vs. HC; #P<0.05 vs. NI; *P<0.05 vs. HI).
C: Histopathological sections of the spleens of mice in each group.
Figure 5 . a: Detection of related inflammatory factors in serum by ELISA (mean±SD, n= 9/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; 0.05 vs. NC; ¤ 0.05 vs. HC; #P<0.05 vs. NI; *P<0.05 vs. HI).
b: Detection of related inflammatory factors in the spleen by ELISA (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; ¤ 0.05 vs. HC; #P<0.05 vs. NI; *P<0.05 vs. HI).
c: Detection of inflammatory factors in the spleen by PCR (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI; *P<0.05 vs. HI).
d: Detection of inflammatory factors in the colon by PCR (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI; *P<0.05 vs. HI).
Figure 6 . a: Detection of phagocytic chemokines in the spleen by PCR (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI; *P<0.05 vs. HI).
b: Detection of phagocytic chemokines in the colon (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI; *P<0.05 vs. HI).
c: Detection of related chemokines in the spleen by WB (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI;*P<0.05 vs. HI).
d: Detection of related chemokines in the colon by WB (mean±SD, n=3/group; & 0.05 vs. NC; ▪ 0.05 vs. HC; *P<0.05 vs. NI; @ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI; *P<0.05 vs. HI).
Figure 7 .: Results of immunofluorescence staining for chemokines in the spleen and colon of mice in each group (protein fluorescence, nuclear fluorescence, and merged coincidence signals,200×). A: MIP2 for WT-spleen. B: MIP2 for MPO-/--spleen. C: KC for WT-spleen. D: KC for MPO-/--spleen. E: MCP1 for WT-spleen. F: MCP1 for MPO-/--spleen. G: MIP2 for WT-colon. H: MIP2 for MPO-/--colon. I: KC for WT-colon. J: KC for MPO-/--colon. K: MCP1 for WT-colon. L: MCP1 for MPO-/--colon.