Figure captions
Figure 1 . Changes in the body weight of mice in each group
(mean±SD, n= 8/group; &P˂ 0.05 NI vs. NC; @P<0.05 KI vs. KC; ¥P<0.05
HKI vs. HKC; +P<0.05 KI vs. NI; #P<0.05 HI vs. HC;
*P<0.05 HKI vs. HI).
Figure 2 . a: Twenty-four-hour results of mouse faecal smear
culture. C. rodentium grew well on MacConkey medium.
b: The number of bacteria excreted by mice in each group (mean ± SD, n=
8/group; *P˂0.05 vs. NI; #P˂ 0.05 vs. KC; +P˂ 0.05 vs. KI;
*P˂ 0.05 vs. HKI).
Figure 3 . a: Photographs of the colons of mice in each group.
Compared with that in the normal group, the colon morphology of the mice
in the infected group changed to varying degrees; the main symptoms were
shortening, and individual mice had colon thinning and ulceration.
b: Changes in the colon length of mice in each group (mean±SD, n=
8/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC;
*P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs.
HKC;+P<0.05 vs. KI; ◊P˂ 0.05 vs. NC;¤P˂ 0.05 vs. HC; #P<0.05 vs. NI;
*P<0.05 vs. HI).
Figure 4 . a: Pathological sections of the colons of mice in
each group. According to the comparison, the intestinal mucosal tissue
damage in the HI and KI groups was more serious, and the main symptoms
were thinning of the intestinal wall, becoming almost transparent, with
more severe oedema, disordered villi with different lengths, and not
only shallower but also a reduced number of crypts. These alterations
were accompanied by a large amount of inflammatory cell
infiltration.(20×)
b: Results of pathological scoring of mice in each group. Compared with
those of the NI and HI groups, the pathological results of the infection
group were more significant with hypoxia (P < 0.05),
indicating that hypoxia can aggravate the mouse pathological lesions of
the colon (mean±SD, n= 8/group; & P˂ 0.05 vs. NC; ▪
P<0.05 vs. HC; *P<0.05 vs. NI; @P <0.05 vs.
KC; ¥P<0.05 vs. HKC;+P<0.05 vs. KI; ◊P˂ 0.05 vs. NC; ¤P˂ 0.05 vs. HC; #P<0.05 vs.
NI; *P<0.05 vs. HI).
C: Histopathological sections of the spleens of mice in each group.
Figure 5 . a: Detection of related inflammatory factors in serum
by ELISA (mean±SD, n= 9/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05
vs. HC; *P<0.05 vs. NI; @P˂ 0.05 vs. KI;
¥P<0.05 vs. HKC; +P<0.05 vs. KI;◊P˂ 0.05 vs. NC; ¤P˂ 0.05 vs. HC;
#P<0.05 vs. NI; *P<0.05 vs. HI).
b: Detection of related inflammatory factors in the spleen by ELISA
(mean±SD, n=3/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC;
*P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs.
HKC; +P<0.05 vs. KI; ¤P˂ 0.05 vs. HC;
#P<0.05 vs. NI; *P<0.05 vs. HI).
c: Detection of inflammatory factors in the spleen by PCR (mean±SD,
n=3/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC;
*P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs.
HKC; +P<0.05 vs. KI; #P<0.05 vs. NI;
*P<0.05 vs. HI).
d: Detection of inflammatory factors in the colon by PCR (mean±SD,
n=3/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC;
*P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs.
HKC; +P<0.05 vs. KI; #P<0.05 vs. NI;
*P<0.05 vs. HI).
Figure 6 . a: Detection of phagocytic chemokines in the spleen
by PCR (mean±SD, n=3/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs.
HC; *P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05
vs. HKC; +P<0.05 vs. KI; #P<0.05 vs. NI;
*P<0.05 vs. HI).
b: Detection of phagocytic chemokines in the colon (mean±SD, n=3/group;
&P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC; *P<0.05 vs.
NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs. HKC; +P<0.05
vs. KI; #P<0.05 vs. NI; *P<0.05 vs. HI).
c: Detection of related chemokines in the spleen by WB (mean±SD,
n=3/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC;
*P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs.
HKC; +P<0.05 vs. KI; #P<0.05 vs.
NI;*P<0.05 vs. HI).
d: Detection of related chemokines in the colon by WB (mean±SD,
n=3/group; &P˂ 0.05 vs. NC; ▪ P˂ 0.05 vs. HC;
*P<0.05 vs. NI; @P˂ 0.05 vs. KI; ¥P<0.05 vs.
HKC; +P<0.05 vs. KI; #P<0.05 vs. NI;
*P<0.05 vs. HI).
Figure 7 .: Results of immunofluorescence staining for
chemokines in the spleen and colon of mice in each group (protein
fluorescence, nuclear fluorescence, and merged coincidence
signals,200×). A: MIP2 for WT-spleen. B: MIP2 for
MPO-/--spleen. C: KC for WT-spleen. D: KC for
MPO-/--spleen. E: MCP1 for WT-spleen. F: MCP1 for
MPO-/--spleen. G: MIP2 for WT-colon. H: MIP2 for
MPO-/--colon. I: KC for WT-colon. J: KC for
MPO-/--colon. K: MCP1 for WT-colon. L: MCP1 for
MPO-/--colon.