3.5 The effect of MPO in the chemotaxis of neutrophils and macrophages of C. rodentium-infected mice under hypoxia
The results showed that compared with those of the control groups (Figure 6 a-d), the expression levels of MIP2, KC and MCP1 in the spleen and colon of each infected group were significantly increased (P <0.05), while in hypoxia, the expression levels of MIP2, KC and MCP1 in the spleen and colon were decreased compared with those in the control condition (P <0.05), which suggested that the neutrophil and macrophage chemotaxis capacity was reduced in hypoxia. The results of fluorescence (Figure 7) quantitative analysis showed that compared with that in the normal groups, the expression of chemokines in the infected groups was significantly increased, and the content of chemokines in the MPO-/-groups was even greater.