3.4 The effect of MPO in inflammatory response of C.
rodentium-infected mice under hypoxia.
Compared with the control mice (Figure 5a-d), the infected mice had
significantly increased expression levels of the inflammatory factors
TNF-α, IFN-γ and IL-1β in the serum, spleen and colon, and the IL-1β
levels in the serum were also significantly increased. The expression of
IL-6 was significantly increased in the serum ( P<0.05), while the expression of the above factors decreased
under hypoxia exposure, which suggests that the immune response was
downregulated under hypoxia (P <0.05). Comparing WT mice
and MPO-/- mice, the content of TNF-α in the KI and
HKI groups was different from that in the NI and HI groups. Comparing
the IL-1β, IFN-γ and IL-6 levels between the KI and HKI groups with
those in the NI and HI groups, there was no significant difference,
which indicated that the MPO gene has no effect on the production of
inflammatory factors in the body.