##Hematoxylin and eosin stanning
Hematoxylin and eosin (HE) staining was used to evaluate the
histological structure of each group. After anesthesia, the rats were
perfused with 0.9% NaCl and 4% paraformaldehyde through their cranium.
Kidney tissue was extracted and soaked in 4% paraformaldehyde at 4 ℃
for one day. Following this, paraffin-embedded, 5-µm thick sections were
stained with HE.