##Reduction of DN inflammation by ferroptosis inhibitor
Ferroptosis is involved in mediating the inflammatory response. The
expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and
intercellular adhesion molecule 1 (ICAM-1) were assessed by Western
blotting post-DN to clarify the content of inflammation (Figure
5A,5B,5C,5D). The IL-1β, TNF-α, and ICAM-1 in the kidneys of rats in the
DN group was significantly increased compared to the control group. SRS
16-86 attenuated the levels of expression. SRS 16-86 decreased the
levels of inflammatory cytokines in the kidneys of rats with DN.
##Improvement of theorganizational structure
after DN via the inhibition of ferroptosis
To explore whether SRS 16-86 modulates the organizational structure
after DN, we examined the kidneys of rats in each group with HE staining
(Figure 6). In comparison to kidneys in the control group, obvious
disorder was visible on the section of the kidneys in the DN group. In
the DN group, the glomerular morphology was abnormal, the renal tubule
space was enlarged, and renal tissue fibrosis was enhanced. Meanwhile,
the DN-SRS group showed an improvement of organizational structure when
compared with the DN group. These results show that SRS 16-86
significantly reduced destruction of the organizational structure as
evidenced by the increased amount of spared normal tissue and the
decreased extent of lesions, which were associated with improved renal
function recovery.