2.6 Sequencing and data analysis
Quality inspection was performed on the formed sequencing library, and processing, including barcode recognition, was performed on the high-quality circular consensus sequencing (CCS) sequence obtained. The generated optimization CCS was clustered at the level of 97% similarity (USEARCH, version 10.0), and its species classification was obtained based on the sequence composition of the operational taxonomic unit (OTU). The platforms 16S: Silva database and RDP Classifier were used to analyze species annotation and taxonomy as well as the diversity of gut microbiota.