microRNA
|
Optical technique
|
Linear dynamic range
|
Disease
|
Mechanism
|
Reference
|
miRNA-21
|
Fluorescence
|
50 pM–1 nM
|
RA
|
Immobilized FAM-labelled hairpin probes on AuNPs; amplification based on
duplex-specific nuclease
|
|
let-7a
|
Fluorescence
|
10 fM–2 pM
|
RA
|
Helicase (RecQE) -assisted hybridization chain reaction on graphene
oxide (GO) platform
|
|
miRNA-155
|
Absorbance
|
100 aM–100 fM
|
RA
|
DNA probe bound to citrate-capped Au nanoparticles; target miRNA
adsorbed on polyethylenimine-capped AuNP surface
|
|
miRNA-15a
|
SPR (Surface Plasmon Resonance)
|
5 fM–0.5 nM
|
RA
|
Isolated Au islands on innovative chip installed on SPRi imager bordered
by hydrophobic fluoropolymer- CYTOP
|
|
miRNA-21, miRNA-155
|
SPR (Surface Plasmon Resonance)
|
10 aM–10 pM
|
RA
|
SPR chip surface coated with antimonene nanosheets; amplification due to
interaction with AuNR-ssDNA
|
|
miRNA-155
|
SERS (Surface Enhanced Raman Spectroscopy)
|
1 fM–10 nM
|
RA
|
SERS enhanced DSN amplification in DNA microcapsule using TB@CaCO3 blend
|
|
miRNA-21
|
square wave voltammetry (SWV); electrochemical
|
200 pM–1 fM
|
T2DM
|
streptavidin-biotin interaction mediated deposition of AgNPs on Au
electrode
|
|
miRNA-155
|
differential pulse voltammetry (DPV); electrochemical
|
0.1 fM–1.0 nM
|
T2DM
|
Mo2C nanosheets depositied with AuNPs based on catalytic
hairpin assembly
|
|
miRNA-375
|
square wave voltammetry (SWV); electrochemical
|
10–30 fM
|
T2DM
|
Immobilized miR-375 capture probe on Au electrode followed by
hybridization
|
|
miRNA-34a
|
electrochemical impedance spectroscopy (EIS)
|
2–10 µg/mL
|
T2DM
|
Immobilized ionic liquid (1-butyl-3-methylimidazolium
hexafluorophosphate) on activated pencil graphite electrodes
|
|
miRNA-145
|
Fluorescence
spectrophotometry
|
N/A
|
MS
|
Hybridization chain reaction based formation of AgNCs in DNA (highly
fluorescent)/use of oligonucleotide hairpin probes
|
|
miRNA- 23a,
|
|
|
|
|
|