GO analyses of Differentially expressed genes.
After comparing and analyzing the GO results for the BP, CC, and MF in the 100μg DNA IM group and 50μg DNA EP group (Supplementary Fig. 2), we found that: (1) 20 of the 30 GO items in down-regulated DE genes and 22 of the 30 GO items in up-regulated DE genes in the two groups are consistent. (2) In the two DNA groups, the down-regulated BP items were mainly enriched in the secretion and regulation of cells, proteins, insulin, hormones, etc, in addition, DNA EP significantly decreased the secretion and regulation of neurotransmitters at the same time; the up-regulated BP items were mainly enriched in the developmental process, etc. The down-regulated CC items were mainly enriched in the neuronal cell body, secretory granule, synapse part, extracellular region, etc; the up-regulated CC items were mainly enriched in the extracellular region, extracellular matrix part, vesicle, etc. The down-regulated MF items were mainly enriched in peptidase activity, calcium ion binding, hormone activity, triglyceride lipase activity, etc; the up-regulated MF items were mainly enriched in binding, protein binding, receptor binding, growth factor binding, ion binding, etc.