Conclusion
MTB infection caused significant up-regulation of catabolism-related DE
genes, GO biological processes, and signal pathways in mice, and
significant down-regulation of anabolism-related DE genes, GO biological
processes, and signal pathways, as well as significant down-regulation
of multiple immune-related pathways. Our study found for the first time
that the effective doses of ag85a/b DNA vaccine immunized whether
by IM or EP could significantly up-regulate immune-related pathways, and
recover the metabolic disorder and the injury caused by MTB. The action
target and mechanism of two effective treatment groups (100μg DNA IM
group and 50μg DNA EP group) are highly consistent. These findings
provide a basis for further elucidating the immunotherapeutic target and
mechanism of the ag85a/b DNA vaccine.