Pilot (HiSeq 150 PE, N = 79) Pilot (HiSeq 150 PE, N = 79) Pilot (HiSeq 150 PE, N = 79) Full (2 x Novaseq 150 PE, N = 456) Full (2 x Novaseq 150 PE, N = 456) Full (2 x Novaseq 150 PE, N = 456)
Step Reads zOTUs Reads zOTUs
Raw PE reads
412 449 403
-
-
5 436 416 402
-
-
Paired, filtered, demultiplexed
342 785 829
-
-
4 857 351 483
-
-
Denoised, without chimeras or singletons (<4)
284 213 421
49 723
-
4 268 556 612
130 677
-
Assigned to taxonomy
284 212 399
49 697
-
4 268 371 437
129 940
-
Sample type POA POA
Extraction negatives (np = 4, nf = 19)
10 801 345
1 986
3.80
8 269 055
5 046
0.19
Real samples (np = 75, nf = 437)
273 411 054
48 252
96.20
4 259 081 079
129 182
99.78
Identified taxa in real samples POA POA
Consumer
267 342 579
43 766
97.78
4 153 774 204
90 725
97.53
Symbionts
587 313
599
0.21
8 160 170
4 266
0.19
Contaminants
4 291 765
2 387
1.57
44 980 919
11 800
1.06
Prey
1 189 397
1500
0.44
52 165 786
22 391
1.22