2.1 Laboratory animal
Sprague-Dawley rats (SD), male, body weight 230-250 g, 12-14 weeks, and were illuminated alternately by 12 h light (temperature 22-24℃, humidity 40%-70%). The experimental animals were purchased from the Laboratory Animal Center of Guizhou Medical University, China, Certificate number: SCXY (Gui)2023-0002, and the experiment was approved by the Experimental Animal Ethics Committee of Guizhou Medical University (approval no. 2303043) and followed the National Institutes of Health Guide for the Care and Use of Laboratory Animals, while following the Guidelines for Ethical Review of animal welfare (Reference NO. GB/T 35892-2018).