2.10 Immunofluorescence detection
The paraffin sections were dewaxed to water by the usual procedure
described above. After sealing, the primary antibody was added directly
without cleaning. The primary antibodies were CD86 (cat. no. 91882; Cell
Signaling; 1:100), CD206 (cat. no. 24595; Cell Signaling; 1:100) and
Iba1 (cat. no. 17198; Cell Signaling; 1:100). Nuclei were labeled with
DAPI from Beyotime Institute of Biotechnology, the issues were sealed
with anti-fluorescence quenched plates and the images were taken with a
confocal laser microscope (Olympus, Japan).