3.5 NSC-Exos mitigated the inflammatory response induced by MCAO model and decreased the expression of Iba1 protein in microglia
​Iba1 is a microglia-specific marker to observe the effect of NSC-Exos therapy on microglia. The expression was detected by immunohistochemistry and western blotting. The results showed as follows: Compared with Sham group, the number of Iba1 positive cells in IRI group and PBS group was increased (P< 0.01), the morphology of Iba1 positive cells was higher than that of hypertrophic cells, and the protein expression level was also increased (P< 0.05). Compared with IRI group and PBS group, the number of Iba1 positive cells in NSC-Exos group was decreased. (P< 0.01, Fig. 4a, b), there were fewer branches and hypertrophic cells, and the expression level of Iba1 protein was also decreased in NSC-Exos group (P< 0.05, Fig. 4c, d).