2.9 Immunohistochemical staining
The expression of the microglial marker Iba1 in the cerebral cortex was observed. Paraffin sections of brain tissue were routinely dewaxed to water, peroxidase inhibited by 3% H2O2(10 min), microwave antigen repair by sodium citrate (20 min), cooled to room temperature, sealed by 5% bovine serum albumin (60 min), incubated overnight with primary antibody. On the second day, the slices were taken out and rewarmed (30 min), DAB color development was photographed for observation (Beijing Solarbio Science & Technology Co., Ltd.).