3.6 NSC-Exos improves the inflammatory response of microglia
The inflammatory response mediated by microglia is an important
post-stroke link. qPCR and western blotting were used to detect M1 and
M2 related cytokine expression in microglia. Western blot results show
that M1 microglia related molecules are significantly activated after
injury. Compared with Sham group, the expressions of IL-1β, IL-6, TNF-α,
CD86 and iNOS in IRI group and PBS group were increased
(P< 0.05). Compared with IRI group and PBS group, the
expression of these corresponding factors was down-regulated in NSC-Exos
group (P< 0.05, Fig. 5a-f). After MCAO treatment, M2
microglia-related molecules were significantly inhibited, and the
expression of Arg1 and CD206 in IRI and PBS groups was decreased
compared with Sham group (P< 0.05). After
transplantation of NSC-Exos, their expression was significantly
increased in the NSC-Exos group compared with the IRI and PBS groups
(P< 0.05, Fig. 6a,b,c). This result was also verified by
the same qPCR, which was consistent with the protein expression level
(Fig. 6d,e,f), indicating that treatment with NSC-Exos could inhibit the
activation of M1 pro-inflammatory and up-regulate the polarization of M2
anti-inflammatory.