2.2 MCAO model
According to the modified Zea Longa’s method (Longa et al., 1989), the
animal model of right Middle cerebral artery occlusion (MCAO) was made.
In SD rats, 3% pentobarbital sodium (0.2 ml/100g) was injected
peritoneally, the neck skin was cut open, the right common carotid
artery (CCA), the external carotid artery (ECA) and the internal carotid
artery (ICA) were exposed, a nylon thread was inserted into the stump of
ECA along the artery bifurcation to the middle cerebral artery, and the
right middle cerebral artery was blocked to establish a focal cerebral
ischemia model in the right cerebral hemisphere. After 60 min, the nylon
thread was gently pulled out to achieve reperfusion, and the body
temperature was maintained until awake (37±0.5°C).