3.5 NSC-Exos mitigated the inflammatory response induced by MCAO
model and decreased the expression of Iba1 protein in microglia
Iba1 is a microglia-specific marker to observe the effect of NSC-Exos
therapy on microglia. The expression was detected by
immunohistochemistry and western blotting. The results showed as
follows: Compared with Sham group, the number of Iba1 positive cells in
IRI group and PBS group was increased (P< 0.01), the
morphology of Iba1 positive cells was higher than that of hypertrophic
cells, and the protein expression level was also increased
(P< 0.05). Compared with IRI group and PBS group, the
number of Iba1 positive cells in NSC-Exos group was decreased.
(P< 0.01, Fig. 4a, b), there were fewer branches and
hypertrophic cells, and the expression level of Iba1 protein was also
decreased in NSC-Exos group (P< 0.05, Fig. 4c, d).