2.4 Fluorescence probe PKH26 labeling and MAP-2 co-localization
detection
NSC-Exos was labeled with the red fluorescent dye PKH26 (Hangzhou Yumei
Biotechnology Co., Ltd.) and the dye working solution was prepared
according to instructions. The exosomes were incubated for 15 min in a
dark environment in a working solution. About 200 μg exosomes were added
with 50 μl working solution. The incubated exosome-dye complex was added
to PBS and well mixed. The nerve cells of PKH26 and MAP-2 were
co-located by immunofluorescence assay. The primary antibody was a
rabbit anti-rat MAP-2 (cat. no. 4542; Cell Signaling) monoclonal
antibody, and the secondary antibody was incubated for 2 h.