3.7 NSC-Exos regulates the polarization of microglia
To further investigate the polarization status of microglia after injury, immunofluorescence staining was used to evaluate the colocalization expression of M1 (CD86/ Iba1) and M2 (CD206/ Iba1). The CD86/ Iba1 staining results of M1 microglia showed that CD86+/Iba1+ colocalization expression was significantly increased in IRI and PBS groups compared with Sham group (P< 0.01). Compared with IRI group and PBS group, CD86+/Iba1+colocalization expression was significantly decreased in NSC-Exos group (P< 0.01, Fig. 7a, c). The CD206/Iba1 staining results of M2 microglia showed that compared with Sham group, CD206+/Iba1+ colocalization expression was increased in IRI and PBS groups (P< 0.01). Compared with IRI group and PBS group, the expression of CD206+/Iba1+colocalization was significantly increased in NSC-Exos group (P< 0.01, Fig. 7b, d).