2.7 Morris water maze
The Morris water maze test was carried out in a black water tank
(diameter: 150 cm, height: 60 cm). Morris software encircles the edge of
the pool, measures the corresponding quadrant of the software at the
edge of the pool, and places an escape platform (diameter: 10 cm) in the
middle of one of the quadrants. The position of the platform was kept
constant during the experiment. The experiment was conducted from 1d to
4d (60 sec), and SD rats were placed in the water tank to explore
freely. If a platform is found within the 60 sec, and the time is
recorded to escape the incubation period; If the platform is not found,
the rats are guided to the platform and remain there for 10 sec to end
the experiment. 5 day is a space exploration test, which is free to
explore after the platform is removed. The memory results, such as
escape delay, swimming distance, target quadrant exploration time, and
movement trajectory, were recorded within the specified time and the
data was analyzed using the Morris water maze image processing system
(Chengdu Taimeng Technology Co., Ltd.).