3.3 NSC-Exos can successfully reach the brain injury area and be successfully taken up by neurons
PKH26 dyes are capable of binding fluorescent dyes with longer lipid tails to lipid regions of the cell membrane. We label exosomes with PKH26 dye and then co-stain them with neuronal cells. The results showed that labeled exosomes (red) were located in the cytoplasm of MAP-2 positive neurons (pink), and a small amount overlapped with the nucleus, indicating successful uptake of exosomes by neuronal cells (Fig. 3a).