2.7 Morris water maze
The Morris water maze test was carried out in a black water tank (diameter: 150 cm, height: 60 cm). Morris software encircles the edge of the pool, measures the corresponding quadrant of the software at the edge of the pool, and places an escape platform (diameter: 10 cm) in the middle of one of the quadrants. The position of the platform was kept constant during the experiment. The experiment was conducted from 1d to 4d (60 sec), and SD rats were placed in the water tank to explore freely. If a platform is found within the 60 sec, and the time is recorded to escape the incubation period; If the platform is not found, the rats are guided to the platform and remain there for 10 sec to end the experiment. 5 day is a space exploration test, which is free to explore after the platform is removed. The memory results, such as escape delay, swimming distance, target quadrant exploration time, and movement trajectory, were recorded within the specified time and the data was analyzed using the Morris water maze image processing system (Chengdu Taimeng Technology Co., Ltd.).