Management of virus-specific IgG data
All EGEA1 and EGEA2 samples were analyzed at the same time. Figure S1
shows a workflow representing the different steps of the management of
IgG variables.
Fluorescence intensities (FI) of three replicated spots were measured
and the median value of triplicate measurements was calculated (raw
data). A calibration of raw data was performed, considering the
calibrator and sample diluent estimated in each analysis run (calibrated
data).
To determine the analytical sensitivity for each antigen, the background
signals of all sample diluent repetitive measurements (n=21) were
calculated as a mean FI values + 3 standard deviation for each antigen.
Values below this background signal were zero (corrected data). For
RV-specific IgG data, summary RV-A, RV-B and RV-C variables were
calculated by the sum of IgG responses to 18, 9 and 10 specific peptides
respectively, as listed in Table S1.