Quantitative PCR
Total RNA was isolated using a NucleoSpin RNA kit (Takara Bio Inc., Shiga, Japan). RNA was reverse transcribed using a PrimeScript RT reagent kit with a gDNA eraser (Takara Bio Inc.). cDNA was amplified with a TB Green Premix Ex Taq II kit (Takara Bio Inc.) using a Thermal Cycler Dice Real Time System (Takara Bio Inc.). All primers were purchased from Takara Bio Inc. The primer sequences are listed in Table S1. Relative mRNA levels were normalized to Gapdh mRNA levels.