Fig. 1 Soil fungal community dilution curves and community variation
characteristics
Phylogenetic and compositional variability
We conducted a phylogenetic analysis of the top 100 species with regard
to relative abundance at the species level (Fig. 2a). According to the
results, the fungal community phylogeny was highly aggregated between
the nonrhizosphere and rhizosphere soils of A. spinulosa . The
rhizosphere soil of A. spinulosa was concentrated in Ascomycota,
Mortierellomycota, and Rozellomycota fungi, and the nonrhizosphere soil
was concentrated in Ascomycota and Chytridiomycota fungi.
LEfSe multilevel species difference discrimination analysis (Fig. 2b)
revealed that the species that differed between the rhizosphere soil
community groups of A. spinulosa were mainly at the genus level,
including Cutaneotrichosporon , Chaetopsina ,Dendrosporium , Tubulicium , Shiraia ,Perenniporia , Simplicillium and Capitofimbria ,Tubulicium , Shiraia , Perenniporia ,Simplicillium , and Capitofimbria , with the abundance of
species observed in the Cutaneotrichosporon group. This was the
primary reason for the variation in soil fungi between A.
spinulosa roots. The main groups of nonrhizospherically differentiated
species were the Sordariomycetes group at the phylum level,
Chaetothyriales and Trechisporales groups at the order level, and the
Herpotrichiellaceae group at the family level.
Note: R indicates A.
spinulosa rhizosphere soil fungi; NO indicates A. spinulosanonrhizosphere soil fungi. (a) Phylogenetic evolutionary tree of soil
fungi based on the species level; (b) LEfSe multilevel species
difference discrimination analysis of the soil fungal communities.