Karyotypes of Acanthodoras species after conventional and molecular cytogenetics techniques: Acanthodoras cf. spinosissimus (a) Giemsa staining; (b) C-banding; (c) FISH with 18S rDNA (pink) and 5S rDNA (green) probes; (d) FISH with U2 snDNA (green) and 5S rDNA (pink) probes. Acanthodoras cataphractus (e) Giemsa staining; (f) C-banding; (g) FISH with 18S rDNA (pink) and 5S rDNA (green) probes; (h) FISH with U2 snDNA (green) and 5S rDNA (pink) probes