FIGURE 4 Yinxieling(YXL) inhibits the magration of Langerhans cell(LC) from epidermal to skin draining lymph nods. A: Gating strategy and flow cytometry scatter plot to identify LCs and magratory FITC+LCs; B: Frequency of MHCII+CD207+ LCs and magratory FITC+LCs. n = 3, repreated at least twice with similar results. Comparing to IMQ group, ***p <0.001.
3.4 Yinxieling(YXL) inhibited the IMQ-induced maturation of langerhans cells (LCs).
Epidermal cells were isolated and the ratio of CD80+LC and CD86+LC was detected at 0h and 72h, respectively, to investigate the regulation effect of YXL on LC maturation. The results showed that after three days of culture, the proportion of mature LC in the YXL group was significantly lower than that in the IMQ group, indicating that YXL could significantly inhibit epidermal LC maturation(p<0.05),as show in FIGURE 5.