2.9 Effect of YXL on antigen presentation of LC
One million cells per staining were incubated with 100 μ L 0.5
mg/mL Dextran fluorescein solution. After incubation at 37℃ for 45min,
the antibodies MHC Class II (I-A/I-E) Monoclonal Antibody, eFluor™ 450
(clone: M5/114.15.2, eBioscience) and BD Pharmingen™ APC Mouse
anti-Mouse CD45.2 (clone: 104, BD Pharmingen) were incubated at 4℃ in
the dark. The ratio of FITC+ LC was detected to
determine the antigen presentation effect of LC.