2.8 Effect of YXL on LC maturation
Epidermal cells were stained with antibodies MHC Class II (I-A/I-E) Monoclonal Antibody, eFluor™ 450 (clone: M5/114.15.2, eBioscience), CD207 (Langerin) Monoclonal Antibody, PE (clone: eBioL31, eBioscience), BD Pharmingen™ FITC Hamster Anti-Mouse CD80 (clone: 16-10A1, BD Pharmingen), and BD Pharmingen™ APC Rat anti-Mouse CD86 (clone:GL1, BD Pharmingen)The ratio of CD80+LC and CD86+LC was detected at 0 h and 72 h after epidermal cells were isolated, and the regulation effect of YXL on LC maturation was investigated.