3.3 Yinxieling(YXL) inhibited the IMQ-induced migration of epidermal langerhans cells (LCs)
Langerhans cell migration from the epidermis to the lymph nodes is necessary for specific T lymphocyte responses. In a mouse model of psoriasis-like skin inflammation induced by IMQ, it promoted the migration of epidermal LC to dermal draining lymph nodes. In order to clarify the role of YXL in regulating LC, the migration of LC was studied. Fluorescein isothiocyanate (FITC) was dissolved in acetone and applied to the back skin of mice. After 24 hours, the skin draining lymph nodes were taken for flow cytometry detection. The results showed that the ratio of FITC+LC was significantly reduced in YXL treated group (p <0.001), indicating that YXL could significantly inhibit the migration of IMQ-induced LC from the epidermis to skin draining lymph nodes, FIGURE 4.