2.8 Effect of YXL on LC maturation
Epidermal cells were stained with
antibodies
MHC Class II (I-A/I-E) Monoclonal Antibody, eFluor™ 450 (clone:
M5/114.15.2, eBioscience), CD207 (Langerin) Monoclonal Antibody, PE
(clone: eBioL31, eBioscience), BD Pharmingen™ FITC Hamster Anti-Mouse
CD80 (clone: 16-10A1, BD Pharmingen), and BD Pharmingen™ APC Rat
anti-Mouse CD86 (clone:GL1, BD Pharmingen)The ratio of
CD80+LC and CD86+LC was detected at
0 h and 72 h after epidermal cells were isolated, and the regulation
effect of YXL on LC maturation was investigated.