FIGURE 5 Yinxieling(YXL) inhibits the maturation of Langerhans cell(LC). A: Gating strategy, flow cytometry scatter plot to identify MHCII+CD207+LCs, CD80+LCs, CD80+LCs and Frequency of these cells on 0 h; B: Gating strategy, flow cytometry scatter plot to identify MHCII+CD207+LCs, CD80+LCs, CD80+LCs and Frequency of these cells on 72 h. n = 3, repreated at least twice with similar results. Comparing to IMQ group, *p <0.05,**p <0.01,***p <0.001.
3.5 Yinxieling(YXL) inhibited antigen presentation of langerhans cells (LCs). Langerhans cells are the most important antigen presenting cells in the skin and play a key role in the pathogenesis of psoriasis. Therefore, flow cytometry was used to detect the antigen presentation effect of LC in the epidermis. As showed in FIGURE.6, the antigen-presenting effect of epidermal LC was significantly inhibited by YXL(p <0.01).