FIGURE
4 Yinxieling(YXL) inhibits the magration of Langerhans cell(LC) from
epidermal to skin draining lymph nods. A: Gating strategy and flow
cytometry scatter plot to identify LCs and magratory
FITC+LCs; B: Frequency of
MHCII+CD207+ LCs and magratory
FITC+LCs. n = 3, repreated at least twice with similar
results. Comparing to IMQ group, ***p <0.001.
3.4
Yinxieling(YXL) inhibited the IMQ-induced maturation of langerhans cells
(LCs).
Epidermal cells were isolated and the ratio of CD80+LC
and CD86+LC was detected at 0h and 72h, respectively,
to investigate the regulation effect of YXL on LC maturation. The
results showed that after three days of culture, the proportion of
mature LC in the YXL group was significantly lower than that in the IMQ
group, indicating that YXL could significantly inhibit epidermal LC
maturation(p<0.05),as show in FIGURE 5.