2.7 Effect of YXL on LC migration
Fluorescein isothiocyanate (FITC) was dissolved in acetone and applied
to the skin on the back of mice. Twenty-four hours later, skin draining
lymph nodes were collected for flow detection. The lymph nodes cells
were stained for cell surface markers with
MHC
Class II (I-A/I-E) Monoclonal Antibody, eFluorâ„¢ 450 (clone: M5/114.15.2,
eBioscience), CD207 (Langerin) Monoclonal Antibody, PE (clone: eBioL31,
eBioscience),, and then the proportion of FITC+LC was observed and
calculated.