2.5 Tissue preparation and histological analysis
On the experimental 8th day, the mice were anesthetized with 1.5% phenobarbital sodium. Skin lesions were cut for histopathological examination. All the skin samples had been fixed in 4% neutral-buffered formaldehyde solution for 48h and processed for paraffin embedding. Sections were stained with Hematoxylin-Eosin (HE).