PC Analysis
The quantification of PC content was carried out using HPLC with a
Shimadzu LC-20AD Prominence HPLC system equipped with an Alltech ELSD
6000 detector. The analysis included precise control of gas flow rate
and ELSD tube temperature. Separation was achieved using an Agilent
Zorbax RX-SIL column, employing a specific mobile phase composition. PC
content was calculated based on standard curve methods, using GPCho
(C16:0/18:1) and GPEtn
(C16:0/18:1) standards (Li et al.,
2020).