Confocal laser scanning microscope (CLSM)
Modifications in the microstructure of emulsions, generated through
different methods, throughout the in vitro digestion process,
were visualized using a confocal laser scanning microscope (LEICA, SP8,
Germany). Before observation, Nile red was utilized to label released
oil, resulting in red fluorescence for the two different emulsions
during in vitro digestion. Simultaneously, water phases were
stained with Nile blue. The confocal images were captured using a 63×
oil immersion objective (Rathnakumar et al., 2023).