Scanning Electron Microscopy (SEM) Analysis
Before observation, the emulsion underwent spray drying using a pilot-scale spray drier (GEA-Niro, FSD-16-N, Germany). The feed rate was set at 20 g/min, and the inlet and outlet air temperatures were maintained at 135 and 95 ℃, respectively. The resulting encapsulated sample was collected in a sterile glass vial directly from the cyclone and stored at -20 ℃ for subsequent analysis.
The morphology of the microcapsules obtained from the KO emulsion was examined using Scanning Electron Microscopy (SEM) (Hitachi, SU8010, Japan). SEM analysis was conducted at an accelerated voltage of 3 kV, and prior to examination, microcapsule samples were coated with a thin layer of sputtered gold to enhance imaging quality.